Surve Manalee V, Apte Shruti, Bhutda Smita, Kamath Kshama G, Kim Kwang S, Banerjee Anirban
Bacterial Pathogenesis Lab, Department of Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai 400076, India.
Division of Pediatric Infectious Diseases, Johns Hopkins University School of Medicine, 200 North Wolfe St, Baltimore, MD, 21287, USA.
Curr Res Microb Sci. 2020 Aug 16;1:62-68. doi: 10.1016/j.crmicr.2020.08.001. eCollection 2020 Sep.
Adoption of an endocytosis route promoting safe intracellular trafficking is a pre-requisite for development of invasive diseases by Streptococcus pneumoniae (SPN). We aim to explore the contribution of various endocytic routes in internalization and survival of SPN in blood brain barrier (BBB), a key event in development of pneumococcal meningitis. Pneumococcal entry and survival in brain endothelial cells were evaluated following treatment with combinations of inhibitors to block multiple endocytosis pathways leaving a single entry port open. Entry of SPN into brain endothelium through a novel dynamin independent pathway dictates a separate downstream trafficking itinerary. This allows SPN to evade lysosomal degradation, potentially promoting safe transit across BBB, leading to development of meningitis.
采用促进安全细胞内运输的内吞途径是肺炎链球菌（SPN）引发侵袭性疾病的先决条件。我们旨在探究各种内吞途径在SPN于血脑屏障（BBB）内化及存活过程中的作用，这是肺炎球菌性脑膜炎发展过程中的关键事件。在用抑制剂组合处理以阻断多个内吞途径而仅留一个进入端口开放后，评估肺炎球菌在脑内皮细胞中的进入及存活情况。SPN通过一种新的不依赖发动蛋白的途径进入脑内皮，这决定了一条独立的下游运输路径。这使得SPN能够逃避溶酶体降解，可能促进其安全穿过血脑屏障，从而导致脑膜炎的发生。
