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甲基化（-）-表没食子儿茶素 3-O-没食子酸酯增强了分裂疫苗的作用，同时上调了 Toll 样受体 5。

Methylated (-)-epigallocatechin 3-O-gallate potentiates the effect of split vaccine accompanied with upregulation of Toll-like receptor 5.

机构信息

Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka, 819-0395, Japan.

Department of Chemical Science and Engineering, Tokyo Institute of Technology, 2-12-1 Ookayama, Meguro, Tokyo, 152-8552, Japan.

出版信息

Sci Rep. 2021 Nov 29;11(1):23101. doi: 10.1038/s41598-021-02346-4.

DOI:10.1038/s41598-021-02346-4
PMID:34845235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8630126/
Abstract

Split-virus vaccine serves as a major countermeasure against influenza virus, but its effectiveness and protective action are not complete. We previously demonstrated the effect of Benifuuki, a green tea cultivar in Japan, on enhancing the split-virus vaccine-elicited immune response. However, little is known about the detail mechanisms. Here, we show that EGCG3"Me intake significantly potentiated the vaccine-elicited hemagglutination inhibition titer increase. Flow cytometry analysis revealed the increased Toll-like receptor 5 (TLR5) expression after EGCG3"Me treatment in lamina propria dendritic cells (LPDCs) and macrophages, which play crucial roles in the humoral immune system. TLR5 expression correlated with the level of interleukin-6 (IL-6)/C-C chemokine type receptor 5, which are important mediators of the humoral immunity. Taken together, In vivo and ex vivo studies showed that EGCG3"Me potentiated the split-virus vaccine-elicited immune response accompanied with the upregulation of TLR5 in intestine and splenocyte macrophages.

摘要

分病毒疫苗是对抗流感病毒的主要对策，但它的效果和保护作用并不完全。我们之前已经证明了日本绿茶品种“Benifuuki”对增强分病毒疫苗引起的免疫反应的作用。然而，其详细机制知之甚少。在这里，我们表明 EGCG3"Me 摄入可显著增强疫苗引起的血凝抑制滴度增加。流式细胞术分析显示，EGCG3"Me 处理后在黏膜固有层树突状细胞 (LPDC) 和巨噬细胞中 TLR5 表达增加，TLR5 在体液免疫系统中起着至关重要的作用。TLR5 表达与白细胞介素 6 (IL-6)/C-C 趋化因子受体 5 的水平相关，IL-6/C-C 趋化因子受体 5 是体液免疫的重要介质。综上所述，体内和体外研究表明，EGCG3"Me 增强了分病毒疫苗引起的免疫反应，同时上调了肠道和脾细胞巨噬细胞中的 TLR5。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/e255875fe09d/41598_2021_2346_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/7edc3f33dddd/41598_2021_2346_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/f214e828d4b6/41598_2021_2346_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/3ae348e512d2/41598_2021_2346_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/dc8890ca0dfc/41598_2021_2346_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/e255875fe09d/41598_2021_2346_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/7edc3f33dddd/41598_2021_2346_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/f214e828d4b6/41598_2021_2346_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/3ae348e512d2/41598_2021_2346_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/dc8890ca0dfc/41598_2021_2346_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0fb6/8630126/e255875fe09d/41598_2021_2346_Fig5_HTML.jpg

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IgG Fc sialylation is regulated during the germinal center reaction following immunization with different adjuvants.免疫接种不同佐剂后，生发中心反应期间 IgG Fc 岩藻糖化受到调节。
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