Department of Microbiology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Nov;71(11). doi: 10.1099/ijsem.0.005097.
A novel bacterium, designated strain CAU 1637, was isolated from a tidal mudflat. Cells of strain CAU 1637 were Gram-stain-negative, aerobic, motile with single flagellum and rod-shaped. The optimum conditions for growth were observed at 30 °C, pH 6.0 and in the presence of 2 % (w/v) NaCl. The respiratory quinone was ubiquinone-10. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1637 was closely related to the genus , with the highest similarity to NRBC 112946 (97.4 %), followed by NRBC 16783 (96.8 %), JCM 19310 (96.4 %), KCTC 52373 (95.8 %) and JCM 10543 (95.3 %). The predominant cellular fatty acids were C 7 11-methyl and summed feature 8 (C 7 and/or C 6). The major polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol. The average nucleotide identity values between the novel isolate and related strains ranged from 71.0 to 76.4 %, and the DNA-DNA hybridization values ranged from 19.3 to 20.3 %. The G+C content was 58.4 mol% and the whole-genome size was 4.6 Mb, which included 17 contigs and 3931 protein-coding genes. Based on the taxonomic data, strain CAU 1637 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAU 1637 (=KCTC 82429=MCCC 1K06080).
一株新的细菌，命名为 CAU 1637 株，从潮汐滩涂中分离得到。CAU 1637 株的细胞呈革兰氏阴性，好氧，单鞭毛，杆状，运动。最佳生长条件为 30℃、pH6.0 和 2%（w/v）NaCl。呼吸醌为泛醌-10。基于 16S rRNA 基因序列的系统发育分析表明，CAU 1637 株与属密切相关，与 NRBC 112946（97.4%）的相似度最高，其次是 NRBC 16783（96.8%）、JCM 19310（96.4%）、KCTC 52373（95.8%）和 JCM 10543（95.3%）。主要细胞脂肪酸为 C7 11-甲基和总和特征 8（C7 和/或 C6）。主要极性脂类由双磷脂酰甘油和磷脂酰甘油组成。新型分离株与相关菌株之间的平均核苷酸同一性值在 71.0%至 76.4%之间，DNA-DNA 杂交值在 19.3%至 20.3%之间。G+C 含量为 58.4mol%，全基因组大小为 4.6Mb，包括 17 个连续体和 3931 个蛋白质编码基因。基于分类学数据，CAU 1637 株代表属的一个新种，提议命名为 sp. nov.。模式菌株为 CAU 1637（=KCTC 82429=MCCC 1K06080）。
