State Key Laboratory of Animal Nutrition, Beijing Engineering Technology Research Center of Raw Milk Quality and Safety Control, College of Animal Science and Technology, China Agricultural University, Beijing, China.
J Appl Microbiol. 2022 Apr;132(4):2583-2593. doi: 10.1111/jam.15392. Epub 2021 Dec 14.
This study aims to investigate the effect of hydroxy-selenomethionine supplementation on the in vitro rumen fermentation characteristics and microorganisms of Holstein cows.
Five fermentation substrates, including control (without selenium supplementation, CON), sodium selenite supplementation (0.3 mg kg DM, SS03), and hydroxy-selenomethionine supplementation (0.3, 0.6 and 0.9 mg kg DM, SM03, SM06 and SM09, respectively) were incubated with rumen fluid in vitro. The results showed that in vitro dry matter disappearance and gas production at 48 h was significantly higher in SM06 than SM03, SS03 and CON; propionate and total volatile fatty acid (VFA) production was higher in SM06 than CON. Moreover, higher species richness of rumen fluid was found in SM06 than others. Higher relative abundance of Prevotella and Prevotellaceae-UCG-003 and lower relative abundance of Ruminococcus-1 were detected in SM06 than CON. Besides, higher relative abundance of Ruminococcaceae_UCG-005 was found in CON than other treatments.
It is observed that 0.6 mg kg DM hydroxy-selenomethionine supplementation could increase cumulative gas production, propionate, and total VFAs production by altering the relative abundance of Prevotella, Prevotellaceae-UCG-003, Ruminococcaceae_UCG-005 and Ruminococcus-1, so that it can be used as a rumen fermentation regulator in Holstein cows.
This study provides an optimal addition ratio of hydroxy-selenomethionine on rumen fermentation and bacterial composition via an in vitro test.
本研究旨在探讨羟基硒蛋氨酸（HS—Met）补充对荷斯坦奶牛体外瘤胃发酵特性和微生物的影响。
用含有硒（0.3mg/kg 干物质，SS03）、HS—Met（0.3、0.6 和 0.9mg/kg 干物质，SM03、SM06 和 SM09）和无硒（CON）的 5 种发酵底物的培养液进行体外发酵试验。结果表明，SM06 组的体外干物质消失率和 48h 气体产量均显著高于 SM03、SS03 和 CON 组；丙酸和总挥发性脂肪酸（VFA）产量在 SM06 组中最高。此外，SM06 组瘤胃液的物种丰富度高于其他组。SM06 组中普雷沃氏菌和普雷沃氏菌科 UCG-003 的相对丰度高于 CON 组，瘤胃球菌 1 的相对丰度低于 CON 组。此外，CON 组中瘤胃球菌科 UCG-005 的相对丰度高于其他处理组。
本研究表明，0.6mg/kg DM HS—Met 补充可通过改变普雷沃氏菌、普雷沃氏菌科 UCG-003、瘤胃球菌科 UCG-005 和瘤胃球菌 1 的相对丰度，增加累积产气量、丙酸和总 VFA 产量，可作为荷斯坦奶牛瘤胃发酵调节剂。
本研究通过体外试验为 HS—Met 对瘤胃发酵和细菌组成的最佳添加比例提供了依据。
