Moreno Candelaria Gonzalez, Luque Andrea Torres, Galvão Klibs N, Otero María C
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina; Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, Florentino Ameghino s/n, CP 4105, Barrio Mercantil, El Manantial, Tucumán, Argentina.
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina.
Res Vet Sci. 2021 Nov 19;142:15-23. doi: 10.1016/j.rvsc.2021.11.007.
Vaginal microenvironment plays a significant role in bovine fertility since its resident microorganisms interact with the host mucosa and constitutes the first barrier against ascending pathogens in the reproductive tract. In this study, the vaginal microbiome of healthy heifers (H) and cows with impaired reproductive performance, metritis complex (MT) or repeat breeders (RB), was assessed using a 16S rRNA gene sequencing approach. Analysis revealed that even though a vaginal microbiological guild (Firmicutes, Bacteroidetes, Proteobacteria, Tenericutes, Fusobacteria and Actinobacteria) was shared among healthy heifers and cows with uterine disease; further analysis at genus level showed significant differences depending on the reproductive health status. The relative abundances of recognized uterine pathogens such as Bacteroidetes, Fusobacterium and Helcococcus were higher in MT when compared with H and RB; therefore, their presence in vagina can be considered as a risk factor for fertility. The present study describes for the first time, the composition of native bacterial communities in the vagina of cows undergoing the repeat breeding syndrome (RBS), and reports an association between this disease and the presence of Porphyromonas and unassigned genera of the Pasteurellaceae family. In addition, this work highlights the bacteria associated with a healthy vagina: genera from the families Lachnospiraceae, Rikenellaceae and the genera Acinetobacter, Bacillus, Oscillospira, CF231 and 5-7NS. Results highlighted herein, signify the potential of the evaluation of the bovine vaginal microbiome to future design therapeutic interventions to improve pregnancy rates however, further research is needed to elucidate the balance of bacterial species resulting in an optimal reproductive health.
阴道微环境在牛的繁殖力中起着重要作用，因为其常驻微生物与宿主黏膜相互作用，并构成了生殖道抵御上行病原体的第一道屏障。在本研究中，使用16S rRNA基因测序方法评估了健康小母牛（H）以及繁殖性能受损、患有子宫炎综合征（MT）或屡配不孕（RB）的母牛的阴道微生物群。分析表明，尽管健康小母牛和患有子宫疾病的母牛之间共享一个阴道微生物群落（厚壁菌门、拟杆菌门、变形菌门、柔膜菌门、梭杆菌门和放线菌门）；但在属水平上的进一步分析显示，根据生殖健康状况存在显著差异。与H和RB相比，MT中公认的子宫病原体如拟杆菌门、梭杆菌属和嗜眠菌属的相对丰度更高；因此，它们在阴道中的存在可被视为繁殖力的一个风险因素。本研究首次描述了患有屡配不孕综合征（RBS）的母牛阴道中天然细菌群落的组成，并报告了这种疾病与卟啉单胞菌属以及巴斯德菌科未分类属的存在之间的关联。此外，这项工作突出了与健康阴道相关的细菌：毛螺菌科、理研菌科的属以及不动杆菌属、芽孢杆菌属、颤螺菌属、CF231和5-7NS属。本文突出的结果表明，评估牛阴道微生物群对于未来设计提高妊娠率的治疗干预措施具有潜力，然而，需要进一步研究以阐明导致最佳生殖健康的细菌物种平衡。
