McGuigan L E, Geczy A F, Prendergast J K, Edmonds J P, Hart H H, Bashir H V
Ann Rheum Dis. 1986 Feb;45(2):144-8. doi: 10.1136/ard.45.2.144.
We have previously shown that antibodies raised in rabbits to certain enteric bacteria will specifically lyse, in a 51Cr release assay, the peripheral blood lymphocytes (PBL) of 80% of HLA-B27 positive patients with ankylosing spondylitis (B27+ AS+) but not the PBL of HLA-B27 positive normal controls (B27+ AS-). Other laboratories have been unable to reproduce these findings. This study was designed to ascertain whether this lack of reproducibility was due to a peculiarity of our B27+ AS+ patients or to technical difficulties in the complement mediated 51Cr release assay. We have shown in this blind study that the PBL of 16 out of 18 B27+ AS+ patients from a New Zealand population were lysed by our antisera but none of the PBL of 20 B27+ AS- normal controls were lysed. The phenomenon of 'cross reactivity' between certain enteric bacteria and B27+ AS+ PBL is not confined to the Sydney AS population.
我们之前已经表明，在兔体内产生的针对某些肠道细菌的抗体，在铬-51释放试验中，会特异性地裂解80%的强直性脊柱炎HLA - B27阳性患者（B27 + AS +）的外周血淋巴细胞（PBL），但不会裂解HLA - B27阳性正常对照者（B27 + AS -）的PBL。其他实验室无法重复这些发现。本研究旨在确定这种无法重复性是由于我们的B27 + AS +患者的特殊性，还是由于补体介导的铬-51释放试验中的技术困难。在这项盲法研究中，我们发现来自新西兰人群的18名B27 + AS +患者中有16名的PBL被我们的抗血清裂解，但20名B27 + AS -正常对照者的PBL均未被裂解。某些肠道细菌与B27 + AS + PBL之间的“交叉反应”现象并不局限于悉尼的强直性脊柱炎患者群体。
