弱精子症和畸形精子症男性精子中 和 转录本含量的评估
Evaluation of and transcript contents in spermatozoa from men with asthenozoospermia and teratozoospermia.
作者信息
Mazaheri Moghaddam Madiheh, Mazaheri Moghaddam Marziyeh, Amini Mohammad, Bahramzadeh Behzad, Baghbanzadeh Amir, Biglari Alireza, Sakhinia Ebrahim
机构信息
Department of Genetics and Molecular Medicine School of Medicine, Zanjan University of Medical Sciences (ZUMS) Zanjan Iran.
Department of Medical Genetics, Faculty of Medicine Tabriz University of Medical Sciences Tabriz Iran.
出版信息
Health Sci Rep. 2021 Nov 23;4(4):e436. doi: 10.1002/hsr2.436. eCollection 2021 Dec.
BACKGROUND AND AIMS
Motility and morphological defects of spermatozoa can cause male infertility. Sperm RNAs are related to sperm quality. They are considered to have clinical values as a biomarker for assessing sperm quality and fertility potential. The annulus, located in the mammalian sperm tail, is required for motility and terminal differentiation of the spermatozoa. SEPT2, 4, 6, 7, and 12 proteins are the main components of the annulus in the sperm tail. The study aimed to evaluate and mRNA contents in the spermatozoa of patients with asthenozoospermia and teratozoospermia.
METHODS
We evaluated transcript levels of and in the sperm samples of 20 asthenozoospermic, 20 teratozoospermic, and 20 normozoospermic samples using quantitative PCR.
RESULTS
The transcript level was significantly decreased in the asthenozoospermia samples compared with the normal group ( = .013). However, was not significantly different between these two groups. The transcript levels of and were not statistically different between teratozoospermic and normozoospermic groups.
CONCLUSION
In conclusion, downregulation of in patients with asthenozoospermia appears to be associated with poor sperm motility.
背景与目的
精子的运动和形态缺陷可导致男性不育。精子RNA与精子质量相关。它们被认为具有作为评估精子质量和生育潜力生物标志物的临床价值。位于哺乳动物精子尾部的环对于精子的运动和终末分化是必需的。SEPT2、4、6、7和12蛋白是精子尾部环的主要成分。本研究旨在评估弱精子症和畸形精子症患者精子中的 和 mRNA含量。
方法
我们使用定量PCR评估了20例弱精子症、20例畸形精子症和20例正常精子症样本的精子样本中 和 的转录水平。
结果
与正常组相比，弱精子症样本中的 转录水平显著降低（ = 0.013）。然而，两组之间 无显著差异。畸形精子症组和正常精子症组之间 和 的转录水平无统计学差异。
结论
总之，弱精子症患者中 的下调似乎与精子活力差有关。
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