Legetth Oscar, Rodhe Johan, Lang Stefan, Dhapola Parashar, Wallergård Mattias, Soneji Shamit
Division of Molecular Hematology, BMC, Lund University, 22690 Lund, Sweden.
Lund Stem Cell Center, Lund University, 22184 Lund, Sweden.
iScience. 2021 Oct 27;24(11):103251. doi: 10.1016/j.isci.2021.103251. eCollection 2021 Nov 19.
Single-cell RNAseq is a routinely used method to explore heterogeneity within cell populations. Data from these experiments are often visualized using dimension reduction methods such as UMAP and tSNE, where each cell is projected in two or three dimensional space. Three-dimensional projections can be more informative for larger and complex datasets because they are less prone to merging and flattening similar cell-types/clusters together. However, visualizing and cross-comparing 3D projections using current software on conventional flat-screen displays is far from optimal as they are still essentially 2D, and lack meaningful interaction between the user and the data. Here we present CellexalVR (www.cellexalvr.med.lu.se), a feature-rich, fully interactive virtual reality environment for the visualization and analysis of single-cell experiments that allows researchers to intuitively and collaboratively gain an understanding of their data.
单细胞RNA测序是一种常用于探索细胞群体异质性的方法。这些实验的数据通常使用降维方法（如UMAP和tSNE）进行可视化，其中每个细胞被投影到二维或三维空间中。三维投影对于更大、更复杂的数据集可能更具信息性，因为它们不太容易将相似的细胞类型/簇合并和平展在一起。然而，在传统的平面屏幕显示器上使用当前软件可视化和交叉比较三维投影远非最佳选择，因为它们本质上仍然是二维的，并且用户与数据之间缺乏有意义的交互。在这里，我们展示了CellexalVR（www.cellexalvr.med.lu.se），这是一个功能丰富、完全交互式的虚拟现实环境，用于单细胞实验的可视化和分析，使研究人员能够直观地、协作地理解他们的数据。
