Ford R J, Kwok D, Quesada J, Sahasrabuddhe C G
Blood. 1986 Mar;67(3):573-7.
Recent studies have shown that normal human T cells contain a high-molecular-weight (mol wt) protein exhibiting B cell growth factor (BCGF) activity. Other studies have shown that virally transformed human B cells also secrete a high-mol-wt BCGF-like molecule in vitro. We have studied neoplastic B cells from patients with untreated hairy cell leukemia (HCL) to ascertain whether such cytoplasmic BCGF activity is present in the tumor cells. Studies on HCL cells from four patients indicated that BCGF-like activity was in fact present in the cytosolic extracts when tested on autochthonous HCL cells as well as on normal BCGF-dependent human B cell lines. Chromatographic analysis indicated that the BCGF activity from HCL cells was similar in mol wt as well as function to the normal T cell-derived cytosolic BCGF activity. These studies suggest that HCL cells contain and, in some cases, secrete a high-mol-wt growth factor that can be autostimulatory and appears to resemble a similar growth factor molecule found in normal human T cells.
近期研究表明，正常人类T细胞含有一种具有B细胞生长因子（BCGF）活性的高分子量蛋白质。其他研究显示，病毒转化的人类B细胞在体外也分泌一种高分子量的类BCGF分子。我们研究了未经治疗的毛细胞白血病（HCL）患者的肿瘤性B细胞，以确定肿瘤细胞中是否存在这种细胞质BCGF活性。对4例患者的HCL细胞研究表明，当以自身HCL细胞以及正常依赖BCGF的人类B细胞系进行检测时，胞质提取物中确实存在类BCGF活性。色谱分析表明，HCL细胞的BCGF活性在分子量和功能上与正常T细胞来源的胞质BCGF活性相似。这些研究提示，HCL细胞含有且在某些情况下分泌一种高分子量生长因子，该因子可自我刺激，且似乎类似于在正常人类T细胞中发现的一种相似生长因子分子。
