Trimpert Jakob, Adler Julia M, Eschke Kathrin, Abdelgawad Azza, Firsching Theresa C, Ebert Nadine, Thao Tran Thi Nhu, Gruber Achim D, Thiel Volker, Osterrieder Nikolaus, Kunec Dusan
Institut für Virologie, Freie Universität Berlin, Berlin, Germany.
Department of Infectious Diseases and Respiratory Medicine, Charité, Universitätsmedizin Berlin, Berlin, Germany.
Sci Adv. 2021 Dec 3;7(49):eabk0172. doi: 10.1126/sciadv.abk0172. Epub 2021 Dec 1.
Vaccines are instrumental and indispensable in the fight against the COVID-19 pandemic. Several recent SARS-CoV-2 variants are more transmissible and evade infection- or vaccine-induced protection. We constructed live attenuated vaccine candidates by large-scale recoding of the SARS-CoV-2 genome and showed that the lead candidate, designated sCPD9, protects Syrian hamsters from a challenge with ancestral virus. Here, we assessed immunogenicity and protective efficacy of sCPD9 in the Roborovski dwarf hamster, a nontransgenic rodent species that is highly susceptible to SARS-CoV-2 and severe COVID-19–like disease. We show that a single intranasal vaccination with sCPD9 elicited strong cross-neutralizing antibody responses against four current SARS-CoV-2 variants of concern, B.1.1.7 (Alpha), B.1.351 (Beta), B.1.1.28.1 (Gamma), and B.1.617.2 (Delta). The sCPD9 vaccine offered complete protection from COVID-19–like disease caused by the ancestral SARS-CoV-2 variant B.1 and the two variants of concern B.1.1.7 and B.1.351.
疫苗在抗击新冠疫情中发挥着重要且不可或缺的作用。最近出现的几种严重急性呼吸综合征冠状病毒2（SARS-CoV-2）变体更具传播性，能够逃避感染或疫苗诱导的保护作用。我们通过对SARS-CoV-2基因组进行大规模重新编码构建了减毒活疫苗候选株，并表明主要候选株sCPD9可保护叙利亚仓鼠免受原始病毒的攻击。在此，我们评估了sCPD9在罗伯罗夫斯基仓鼠（一种对SARS-CoV-2和严重的类新冠疾病高度易感的非转基因啮齿动物物种）中的免疫原性和保护效果。我们发现，用sCPD9进行单次鼻内接种可引发针对四种当前值得关注的SARS-CoV-2变体，即B.1.1.7（阿尔法）、B.1.351（贝塔）、B.1.1.28.1（伽马）和B.1.617.2（德尔塔）的强烈交叉中和抗体反应。sCPD9疫苗为预防由原始SARS-CoV-2变体B.1以及两种值得关注的变体B.1.1.7和B.1.351引起的类新冠疾病提供了完全保护。
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