Wilson F D, Gershwin M E, Shifrine M, Graham R
Dev Comp Immunol. 1977 Oct;1(4):373-84. doi: 10.1016/s0145-305x(77)80020-7.
Clonogenic populations from bone marrow and spleen of nude mice and their normal littermates were enumerated in vitro using a methylcellulose supported culture system. This technique allows for the simultaneous quantitation of progenitors of granulocyte-monocyte pathways (colony forming units in culture, CFU-C) and for progenitors of "mesenchymal" elements (plaque-forming units in culture or PFU-C). These populations were distinguished in culture by their growth, characteristics and morphology. CFU-C gave rise to suspended colonies of granulocyte-monocyte composition while PFU-C formed surface-adherent colonies of mesenchymal morphological features (fibroblastic and reticuloendothelial morphology). Significant elevations in the relative and absolute numbers of CFU-C and PFU-C were observed in the bone marrow and spleen of 6 wk old nu/nu mice relative to heterozygous littermates. The results are discussed in terms of non-T cells components involved in cell-medited immunity against neoplastic development.
利用甲基纤维素支持的培养系统，在体外对裸鼠及其正常同窝幼鼠骨髓和脾脏中的克隆形成群体进行计数。该技术可同时对粒细胞 - 单核细胞途径的祖细胞（培养中的集落形成单位，CFU - C）和“间充质”成分的祖细胞（培养中的斑块形成单位或PFU - C）进行定量。这些群体在培养中通过其生长、特征和形态加以区分。CFU - C形成粒细胞 - 单核细胞组成的悬浮集落，而PFU - C形成具有间充质形态特征（成纤维细胞和网状内皮形态）的贴壁集落。相对于杂合子同窝幼鼠，在6周龄nu/nu小鼠的骨髓和脾脏中观察到CFU - C和PFU - C的相对数量和绝对数量显著升高。就参与针对肿瘤发生的细胞介导免疫的非T细胞成分对结果进行了讨论。
