Research Institute of Human Morphology, Moscow, Russia.
A. N. Severtsov Institute of Ecology and Evolution, Russian Academy of Science, Moscow, Russia.
Bull Exp Biol Med. 2021 Dec;172(2):210-213. doi: 10.1007/s10517-021-05364-4. Epub 2021 Dec 2.
We studied the dynamic of proliferative activity of cultured mouse transformed fibroblast-like L-929 cells in the logarithmic growth phase. During a long period (December 5-23, 2020), we revealed a 4-day rhythm of daily increase in the number of L-929 cells with an amplitude not lower than in a culture of embryonic fibroblast-like cells. Hence, the formation of the 4-day rhythm is not associated with the molecular mechanisms of inhibition of proliferation, which are absent in transformed cells. Daily thawing of samples of one culture over 17 days showed the presence of a 4-day rhythm synchronous between all thawed samples and the control cell culture. As deep freezing leads to the cessation of all life processes in cells, the formation of a 4-day rhythm of proliferative activity of cell culture is determined by an exogenous mechanism.
我们研究了对数生长期培养的小鼠转化成纤维细胞样 L-929 细胞增殖活性的动态变化。在很长一段时间（2020 年 12 月 5 日至 23 日）内，我们发现 L-929 细胞的数量每天都在增加，呈现出 4 天的周期性，其振幅不低于胚胎成纤维细胞样细胞的培养。因此，这种 4 天的节律形成与转化细胞中不存在的增殖抑制的分子机制无关。在 17 天内每天解冻一个培养物的样本显示，所有解冻的样本与对照细胞培养之间存在同步的 4 天节律。由于深冻会导致细胞内所有生命过程停止，因此细胞培养增殖活性的 4 天节律的形成是由外源性机制决定的。
