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本文引用的文献

1
Detection of three pandemic causing coronaviruses from non-respiratory samples: systematic review and meta-analysis.从非呼吸道样本中检测三种引发大流行的冠状病毒：系统评价和荟萃分析。
Sci Rep. 2021 Aug 9;11(1):16131. doi: 10.1038/s41598-021-95329-4.
2
Evaluation of the analytical performance and specificity of a SARS-CoV-2 transcription-mediated amplification assay.评估一种 SARS-CoV-2 转录介导扩增检测方法的分析性能和特异性。
J Virol Methods. 2021 Aug;294:114182. doi: 10.1016/j.jviromet.2021.114182. Epub 2021 May 10.
3
Tools and Techniques for Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)/COVID-19 Detection.用于严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）/COVID-19 检测的工具和技术。
Clin Microbiol Rev. 2021 May 12;34(3). doi: 10.1128/CMR.00228-20. Print 2021 Jun 16.
4
High throughput detection and genetic epidemiology of SARS-CoV-2 using COVIDSeq next-generation sequencing.高通量检测和 SARS-CoV-2 的遗传流行病学研究：使用 COVIDSeq 下一代测序技术。
PLoS One. 2021 Feb 17;16(2):e0247115. doi: 10.1371/journal.pone.0247115. eCollection 2021.
5
Molecular Diagnostic Tools for the Detection of SARS-CoV-2.用于检测 SARS-CoV-2 的分子诊断工具。
Int Rev Immunol. 2021;40(1-2):143-156. doi: 10.1080/08830185.2020.1871477. Epub 2021 Jan 13.
6
Nucleic Acid-Based Diagnostic Tests for the Detection SARS-CoV-2: An Update.用于检测严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的基于核酸的诊断测试：最新进展
Diagnostics (Basel). 2021 Jan 1;11(1):53. doi: 10.3390/diagnostics11010053.
7
Fully automated detection and differentiation of pandemic and endemic coronaviruses (NL63, 229E, HKU1, OC43 and SARS-CoV-2) on the hologic panther fusion.在 hologic panther fusion 系统上全自动检测和区分大流行冠状病毒和地方性冠状病毒（NL63、229E、HKU1、OC43 和 SARS-CoV-2）。
J Med Virol. 2021 Jul;93(7):4438-4445. doi: 10.1002/jmv.26749.
8
Detecting SARS-CoV-2 at point of care: preliminary data comparing loop-mediated isothermal amplification (LAMP) to polymerase chain reaction (PCR).即时护理点检测 SARS-CoV-2：比较环介导等温扩增（LAMP）与聚合酶链反应（PCR）的初步数据。
BMC Infect Dis. 2020 Oct 20;20(1):783. doi: 10.1186/s12879-020-05484-8.
9
Use of the variplex™ SARS-CoV-2 RT-LAMP as a rapid molecular assay to complement RT-PCR for COVID-19 diagnosis.使用 variplex™ SARS-CoV-2 RT-LAMP 作为一种快速分子检测方法，与 RT-PCR 互补，用于 COVID-19 诊断。
J Clin Virol. 2020 Nov;132:104616. doi: 10.1016/j.jcv.2020.104616. Epub 2020 Aug 31.
10
A Comparison of Whole Genome Sequencing of SARS-CoV-2 Using Amplicon-Based Sequencing, Random Hexamers, and Bait Capture.基于扩增子的测序、随机六聚体和诱饵捕获的 SARS-CoV-2 全基因组测序比较。
Viruses. 2020 Aug 15;12(8):895. doi: 10.3390/v12080895.

SARS-CoV-2 实验室检测方法和技术应用的进展。

Advances in laboratory detection methods and technology application of SARS-CoV-2.

机构信息

The Children's Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children's Regional Medical Center, Hangzhou, China.

出版信息

J Med Virol. 2022 Apr;94(4):1357-1365. doi: 10.1002/jmv.27494. Epub 2021 Dec 10.

DOI:10.1002/jmv.27494
PMID:34854101
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9015480/
Abstract

At present, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is raging worldwide, and the coronavirus disease 2019 outbreak caused by SARS-CoV-2 seriously threatens the life and health of all humankind. There is no specific medicine for novel coronavirus yet. So, laboratory diagnoses of novel coronavirus as soon as possible and isolation of the source of infection play a vital role in preventing and controlling the epidemic. Therefore, selecting appropriate detection techniques and methods is particularly important to improve the efficiency of disease diagnosis and treatment and to curb the outbreak of infectious diseases. In this paper, virus nucleic acid, protein, and serum immunology were reviewed to provide a reference for further developing virus detection technology to provide better prevention and treatment strategies and research ideas for clinicians and researchers.

摘要

目前，严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）正在全球肆虐，由 SARS-CoV-2 引起的 2019 年冠状病毒病严重威胁着全人类的生命和健康。目前还没有针对新型冠状病毒的特效药。因此，尽快对新型冠状病毒进行实验室诊断，并对感染源进行隔离，对于防控疫情至关重要。因此，选择合适的检测技术和方法对于提高疾病诊断和治疗的效率，遏制传染病的爆发尤为重要。本文综述了病毒核酸、蛋白质和血清免疫学，以期为进一步开发病毒检测技术提供参考，为临床医生和研究人员提供更好的防治策略和研究思路。