Institute of Eco-environment and Industrial Technology, Shanxi Agricultural University, Taiyuan 030031, China.
College of Agricultural Economics and Management, Shanxi Agricultural University, Taiyuan 030006, China.
Molecules. 2023 Jul 7;28(13):5280. doi: 10.3390/molecules28135280.
When added to mushroom growing substrates, edible and medicinal herbs affect the mushrooms' nutritional and medicinal value. In this study, polysaccharides (POP-I and POP-I) were extracted and purified from oyster mushrooms grown on substrates supplemented with 0% and 15% Astragalus roots (P and P), respectively, and their chemical structure and immunobiological activities were compared. POP-I and POP-I were extracted using ultrasound-assisted hot water and deproteinized with the Sevage method, depigmented with 30% HO, desalted with dialysis, and purified using DEAE-52 cellulose and Sephadex G-100 dextran column chromatography. The molecular weight of POP-I and POP-I was 21,706.96 and 20,172.65 Da, respectively. Both were composed of monosaccharides -mannose, galacturonic acid, -glucose, -galactose, and -arabinose but in different molar ratios, and both were connected by a pyranoside linkage. POP-I consisted of higher contents of mannose, glucose, galactose and arabinose and lower content of galacturonic acid as compared to POP-I. Both POP-I and POP-I induced NO and TNF-α production but did not show cytotoxic effect or induce ROS generation in RAW264.7 cells. POP-I showed a stronger ability to promote NO and TNF-α production relative to POP-I. In vitro experiments showed that the immunomodulatory activity of POP-I and POP-I in RAW264.7 macrophages were mediated by the JNK/MAPK, Erk/MAPK, and NF-κB signaling pathways. The results would be helpful for elucidation of the health promoting mechanism of Astragalus oyster mushrooms as a source of neutraceuticals.
当添加到蘑菇生长基质中时，食用和药用草药会影响蘑菇的营养和药用价值。在这项研究中，从分别用 0%和 15%黄芪根（P 和 P）补充的基质中生长的牡蛎蘑菇中提取和纯化了多糖（POP-I 和 POP-I），并比较了它们的化学结构和免疫生物学活性。使用超声辅助热水提取 POP-I 和 POP-I，并用 Sevage 法脱蛋白，用 30%HO 脱色，用透析脱盐，并用 DEAE-52 纤维素和 Sephadex G-100 葡聚糖柱色谱纯化。POP-I 和 POP-I 的分子量分别为 21706.96 和 20172.65 Da。两者均由单糖 -甘露糖、半乳糖醛酸、-葡萄糖、-半乳糖和 -阿拉伯糖组成，但摩尔比不同，均通过吡喃糖苷键连接。与 POP-I 相比，POP-I 含有更高含量的甘露糖、葡萄糖、半乳糖和阿拉伯糖，而半乳糖醛酸含量较低。POP-I 和 POP-I 均诱导 NO 和 TNF-α 的产生，但在 RAW264.7 细胞中没有显示细胞毒性作用或诱导 ROS 生成。与 POP-I 相比，POP-I 显示出更强的促进 NO 和 TNF-α产生的能力。体外实验表明，POP-I 和 POP-I 在 RAW264.7 巨噬细胞中的免疫调节活性是通过 JNK/MAPK、Erk/MAPK 和 NF-κB 信号通路介导的。这些结果将有助于阐明黄芪牡蛎蘑菇作为营养保健品的健康促进机制。
