Simmons J G, Hutt-Fletcher L M, Fowler E, Feighny R J
J Immunol. 1983 Mar;130(3):1303-8.
Raji, a human lymphoblastoid cell line, expresses a membrane receptor (EBVR) specific for Epstein Barr virus (EBV). A component that binds EBV was extracted from this cell line by treatment of the cells for 3 hr on ice with Tris buffer containing 10% glycerol. The treatment reduced the capacity of the cells to bind virus, and after concentration the receptor extract (RE) inhibited both EBV binding and superinfection of fresh Raji cells. Similarly prepared extracts of EBVR- cells lacked such activity. An antibody was made to the extract (anti-RE), which after absorption with EBVR- cells, bound to the same percentages of EBVR+ lymphoblastoid cell lines, EBVR+ human/mouse somatic cell hybrids, and fresh peripheral B cells as the virus did. In reciprocal assays, preincubation of EBVR+ cells with anti-RE inhibited virus binding. Doubly stained patches were observed on membranes of EBVR+ cells that had been incubated simultaneously with virus and anti-RE and stained respectively with rhodaminated and fluoresceinated reagents. The major polypeptide immunoprecipitated by anti-RE from radiolabeled Raji cells had an approximate calculated m.w. of 150,000.
拉吉细胞系是一种人淋巴母细胞系，表达一种针对爱泼斯坦 - 巴尔病毒（EBV）的膜受体（EBVR）。通过用含10%甘油的Tris缓冲液在冰上处理该细胞系3小时，从其中提取出一种能结合EBV的成分。这种处理降低了细胞结合病毒的能力，浓缩后的受体提取物（RE）能抑制新鲜拉吉细胞的EBV结合和超感染。同样制备的EBVR - 细胞提取物则缺乏这种活性。制备了针对该提取物的抗体（抗 - RE），在用EBVR - 细胞吸收后，它与EBVR + 淋巴母细胞系、EBVR + 人/鼠体细胞杂种以及新鲜外周B细胞结合的百分比与病毒相同。在反向试验中，用抗 - RE预孵育EBVR + 细胞可抑制病毒结合。在与病毒和抗 - RE同时孵育并分别用罗丹明标记和荧光素标记试剂染色的EBVR + 细胞的膜上观察到了双重染色斑块。抗 - RE从放射性标记的拉吉细胞中免疫沉淀出的主要多肽，其计算分子量约为150,000。
