Marche P N, Kindt T J
Proc Natl Acad Sci U S A. 1986 Apr;83(7):2190-4. doi: 10.1073/pnas.83.7.2190.
Information relevant to allelic exclusion in T cells has been obtained by a study of cDNA clones corresponding to alpha-chain genes of the T-cell receptor in the rabbit T-cell line RL-5. One clone contains a variable-joining-constant (VJC) sequence encoding a complete alpha chain of the T-cell receptor. A second has an identical constant region and includes a distinct variable-joining (VJ) sequence. However, a single-base deletion in the variable region places the remainder of the second transcript out-of-phase and appears to be the product of a rearrangement involving a variable region of the T-cell receptor alpha-chain pseudogene. Presence of two variable-joining-constant (VJC) transcripts in the same cell line indicates that alpha-chain gene rearrangement is not affected by transcription of a complete alpha-chain mRNA and suggests that steps after mRNA synthesis are involved in the allelic exclusion process for alpha-chain genes. Comparison of rabbit alpha-chain sequences with those of man and mouse revealed interspecies conservation in constant and variable regions. Genomic Southern blot analyses using a rabbit constant region of the T-cell receptor alpha-chain probe revealed the presence of a single constant region gene. Hybridization with variable region probes defined two distinct multigenic subfamilies. Homology between certain rabbit and murine variable regions of the T-cell receptor alpha-chain sequences suggests that the existence of subfamilies predated divergence of these species.
通过对兔T细胞系RL - 5中与T细胞受体α链基因相对应的cDNA克隆进行研究,已获得了与T细胞等位基因排斥相关的信息。一个克隆包含一个编码T细胞受体完整α链的可变 - 连接 - 恒定(VJC)序列。另一个具有相同的恒定区,并包含一个不同的可变 - 连接(VJ)序列。然而,可变区中的一个单碱基缺失使第二个转录本的其余部分相位不一致,似乎是涉及T细胞受体α链假基因可变区的重排产物。同一细胞系中存在两种可变 - 连接 - 恒定(VJC)转录本表明α链基因重排不受完整α链mRNA转录的影响,并提示mRNA合成后的步骤参与了α链基因的等位基因排斥过程。将兔α链序列与人及小鼠的序列进行比较,发现在恒定区和可变区存在种间保守性。使用兔T细胞受体α链恒定区探针进行的基因组Southern印迹分析显示存在单个恒定区基因。与可变区探针杂交确定了两个不同的多基因亚家族。兔和小鼠T细胞受体α链序列某些可变区之间的同源性表明,这些亚家族的存在早于这些物种的分化。