Laboratory of Hematology, Luis Calvo Mackenna Hospital, Santiago, Chile.
Int J Lab Hematol. 2022 Jun;44(3):461-467. doi: 10.1111/ijlh.13776. Epub 2021 Dec 2.
Reticulocytes are erythroid precursors that develop into mature erythrocytes, and they are an important tool to assess erythropoietic activity, as their count indicates the balance between the cells released from the bone marrow, their stage of maturity, and their rate of development into mature erythrocytes. Considering the described biological variability of the absolute reticulocyte count (ARC) and the immature reticulocyte fraction (IRF) and the limited information available on these hematological parameters in children, this study determined the reference intervals (RIs) of these parameters in a healthy pediatric population.
A retrospective, observational, and analytical study was designed to establish RIs for the ARC and the IRF according to age and sex. An indirect sampling method was applied to a mixed database of complete blood counts from children aged 2 months to 18 years, using the truncated maximum likelihood indirect method for reference interval estimation. Percentiles were calculated to obtain bimodal RIs.
From a total of 190,812 samples, 6,814 were selected. Gender stratification was not necessary for the ARC and the IRF but they required partitioning into six and two age groups, respectively.
This study determined, by an indirect sampling method, RIs for the ARC and the IRF in a pediatric population according to age and sex.
网织红细胞是红细胞的前体细胞，可发育为成熟红细胞，其计数可评估红细胞生成活性，因为其计数可反映骨髓释放细胞、细胞成熟度和向成熟红细胞发育的速度之间的平衡。鉴于绝对网织红细胞计数（ARC）和未成熟网织红细胞分数（IRF）的描述性生物学变异性以及这些血液学参数在儿童中的信息有限，本研究旨在确定健康儿科人群中这些参数的参考区间（RI）。
本研究为回顾性、观察性和分析性研究，旨在根据年龄和性别确定 ARC 和 IRF 的 RI。采用截断最大似然间接法间接取样法，对年龄在 2 个月至 18 岁的儿童的全血细胞计数混合数据库进行了间接取样法，以估计参考区间。计算百分位数以获得双峰 RI。
从总共 190812 个样本中，选择了 6814 个样本。ARC 和 IRF 不需要进行性别分层，但需要分别划分为 6 个和 2 个年龄组。
本研究通过间接取样法，根据年龄和性别确定了儿科人群中 ARC 和 IRF 的 RI。
