Ziegler A, Heinig J, Müller C, Götz H, Thinnes F P, Uchańska-Ziegler B, Wernet P
Immunobiology. 1986 Mar;171(1-2):77-92. doi: 10.1016/S0171-2985(86)80019-5.
The monoclonal antibodies (MOABs) TU22, TU34, TU35, TU36, TU37, TU39, TU43, TU58 and YD1/63.HLK were used to identify subpopulations of class II antigens encoded by the human major histocompatibility complex. Since all MOABs reacted with B lymphocytes of HLA-DR1-8 homozygous as well as all heterozygous cells tested, they recognize monomorphic determinants, with the possible exception of TU58 and YD1/63.HLK which do not fix complement. As shown by radioactive binding assays and immunoprecipitations of labeled chains, 3 MOABs reacted strongly and 3 others weakly with isolated beta-chains, and the former also bound alpha-chains, albeit very weakly. Immunoprecipitations with the MOABs from 125I-labeled KR3598 cells (Dw5, DR5, MT2, MB3 homozygous, SB2, SB4) demonstrated that at least 4 different subpopulations of class II antigens were present in the lysate. Possibilities to reconcile these biochemical data with the reactivity of the MOABs with HLA mutant cell lines and with functional as well as tissue distribution studies are discussed.
单克隆抗体（MOABs）TU22、TU34、TU35、TU36、TU37、TU39、TU43、TU58和YD1/63.HLK被用于鉴定人类主要组织相容性复合体编码的II类抗原亚群。由于所有单克隆抗体均与HLA-DR1 - 8纯合以及所有测试的杂合细胞的B淋巴细胞发生反应，因此它们识别单态决定簇，可能不包括不固定补体的TU58和YD1/63.HLK。放射性结合分析和标记链的免疫沉淀结果表明，3种单克隆抗体与分离的β链反应强烈，另外3种反应较弱，前者也能结合α链，尽管结合非常弱。用来自125I标记的KR3598细胞（Dw5、DR5、MT2、MB3纯合，SB2、SB4）的单克隆抗体进行免疫沉淀表明，裂解物中至少存在4种不同的II类抗原亚群。本文讨论了将这些生化数据与单克隆抗体与HLA突变细胞系的反应性以及功能和组织分布研究结果相协调的可能性。
