Calvert J E, Calogeras A
Immunology. 1986 May;58(1):37-41.
Non-T cells from tonsil or blood were fractionated according to buoyant density, isotype of surface immunoglobulin, or the ability to form rosettes with mouse erythrocytes. Each fraction was tested for the ability to proliferate in response to B. catarrhalis (Bc) and, for comparison, Staphylococcus aureus Cowan 1 (SAC) or an MLR supernatant (TF). Cells in all density fractions responded to Bc, the greatest response occurring in the high-density cell fraction. SAC could similarly induce proliferation in high-density cells, in contrast to TF which preferentially activated cells in the low-density fraction. When cells were fractionated by rosetting with mouse erythrocytes, both fractions (MRBC-R+ and MRBC-R-) responded to Bc and to SAC, whereas the greatest response to TF occurred in the MRBC-R- cell fraction. Depletion of sIgD+ sIgM+ cells almost completely abolished the response to Bc, suggesting that responsive cells express both these classes of immunoglobulin on their membrane. Furthermore inclusion of anti-delta antibodies in cultures resulted in failure to proliferate in response to Bc. These data strongly suggest that Bc, like SAC but in contrast to TF, is able to stimulate proliferation in cells with the characteristics of resting B cells, i.e., high-density, sIgD+ cells which form rosettes with MRBC. This may be related to the fact that Bc, like SAC, is able to bind to human immunoglobulins.
来自扁桃体或血液的非T细胞根据浮力密度、表面免疫球蛋白的同种型或与小鼠红细胞形成玫瑰花结的能力进行分离。检测每个分离组分对卡他莫拉菌（Bc）的增殖能力，并作为对照，检测对金黄色葡萄球菌考恩1株（SAC）或混合淋巴细胞反应上清液（TF）的增殖能力。所有密度组分中的细胞均对Bc有反应，最大反应出现在高密度细胞组分中。与优先激活低密度组分细胞的TF相反，SAC同样能诱导高密度细胞增殖。当细胞通过与小鼠红细胞形成玫瑰花结进行分离时，两个组分（MRBC-R+和MRBC-R-）均对Bc和SAC有反应，而对TF的最大反应出现在MRBC-R-细胞组分中。sIgD+sIgM+细胞的耗竭几乎完全消除了对Bc的反应，这表明反应性细胞在其膜上表达这两类免疫球蛋白。此外，在培养物中加入抗δ抗体导致对Bc无增殖反应。这些数据强烈表明，Bc与SAC一样，但与TF不同，能够刺激具有静息B细胞特征的细胞增殖，即高密度、能与MRBC形成玫瑰花结的sIgD+细胞。这可能与Bc和SAC一样能够与人免疫球蛋白结合这一事实有关。
