Transcriptome-Wide Map of N-Methyladenosine Methylome Profiling in Human Bladder Cancer.

N-Methyladenosine (mA) is the most widespread internal RNA modification in several species. In spite of latest advances in researching the biological roles of mA, its function in the development and progression of bladder cancer remains unclear. In this study, we used MeRIPty -55-seq and RNA-seq methods to obtain a comprehensive transcriptome-wide mA profiling and gene expression pattern in bladder cancer and paired normal adjacent tissues. Our findings showed that there were 2,331 hypomethylated and 3,819 hypermethylated mRNAs, 32 hypomethylated and 105 hypermethylated lncRNAs, and 15 hypomethylated and 238 hypermethylated circRNAs in bladder cancer tissues compared to adjacent normal tissues. Furthermore, mA is most often harbored in the coding sequence (CDS), with some near the start and stop codons between two groups. Functional enrichment analysis revealed that differentially methylated mRNAs, lncRNAs, and circRNAs were mostly enriched in transcriptional misregulation in cancer and TNF signaling pathway. We also found that different mA methylation levels of gene might regulate its expression. In summary, our results for the first time provide an mA landscape of human bladder cancer, which expand the understanding of mA modifications and uncover the regulation of mRNAs, lncRNAs, and circRNAs through mA modification in bladder cancer.