Departmant of Urology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Front Cell Infect Microbiol. 2021 Nov 17;11:723781. doi: 10.3389/fcimb.2021.723781. eCollection 2021.
Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the most common type of urinary stones. However, the profile of urinary tract microorganisms of calcium oxalate stones with UTI is not clear. In this research, we firstly found two novel clusters in patients with calcium oxalate stones (OA) that were associated with the WBC/HP (white blood cells per high-power field) level in urine. Two clusters in the OA group (OA1 and OA2) were distinguished by the key microbiota Firmicutes and Enterobacteriaceae. We found that Enterobacteriaceae enriched in OA1 cluster was positively correlated with several infection-related pathways and negatively correlated with a few antibiotics-related pathways. Meantime, some probiotics with higher abundance in OA2 cluster such as Bifidobacterium were positively correlated with antibiotics-related pathways, and some common pathogens with higher abundance in OA2 cluster such as Enterococcus were positively correlated with infection-related pathways. Therefore, we speculated that as a sub-type of OA disease, OA1 was caused by Enterobacteriaceae and the lack of probiotics compared with OA2 cluster. Moreover, we also sequenced urine samples of healthy individuals (CK), patients with UTI (I), patients with uric acid stones (UA), and patients with infection stones (IS). We identified the differentially abundant taxa among all groups. We hope the findings will be helpful for clinical treatment and diagnosis of urinary stones.
尿路结石和尿路感染（UTI）是泌尿外科最常见的疾病，其在中国具有发病率和复发率高的特点。先前的研究表明，尿路结石与肠道或尿液微生物群密切相关。草酸钙结石是最常见的尿路结石类型。然而，UTI 伴草酸钙结石患者的尿路微生物群特征尚不清楚。在这项研究中，我们首次在草酸钙结石（OA）患者中发现了两个与尿液中白细胞/高倍视野（WBC/HP）水平相关的新簇。OA 组中的两个簇（OA1 和 OA2）通过关键微生物群Firmicutes 和肠杆菌科来区分。我们发现，OA1 簇中富集的肠杆菌科与几个感染相关通路呈正相关，与几个抗生素相关通路呈负相关。同时，OA2 簇中一些丰度较高的益生菌，如双歧杆菌，与抗生素相关通路呈正相关，而 OA2 簇中一些丰度较高的常见病原体，如肠球菌，与感染相关通路呈正相关。因此，我们推测，作为 OA 疾病的一个亚型，OA1 是由肠杆菌科引起的，与 OA2 簇相比缺乏益生菌。此外，我们还对健康个体（CK）、UTI 患者（I）、尿酸结石患者（UA）和感染性结石患者（IS）的尿液样本进行了测序。我们确定了所有组之间差异丰度的分类群。我们希望这些发现将有助于尿路结石的临床治疗和诊断。
