一种通过消除抗组蛋白抗体干扰来改进的抗天然DNA酶联免疫吸附测定法。
An improved ELISA for anti-native DNA by elimination of interference by anti-histone antibodies.
作者信息
Rubin R L, Joslin F G, Tan E M
出版信息
J Immunol Methods. 1983 Oct 28;63(3):359-66. doi: 10.1016/s0022-1759(83)80009-x.
We evaluated an enzyme-linked immunosorbent assay (ELISA) for antibodies to native DNA (nDNA) in which protamine was used to link DNA to polystyrene. Elevated anti-nDNA was largely restricted to patients with systemic lupus erythematosus (SLE), and within this group good correlation between ELISA and the ammonium sulfate assay was obtained. However, substantial background immunoglobulin binding to protamine coated wells was commonly observed, and it was necessary to subtract this activity from each anti-DNA determination. Many of the SLE sera also contained anti-histone antibodies, and this antibody activity showed significant correlation with the binding to protamine. In contrast, methylated bovine serum albumin (mBSA) did not bind anti-histone antibodies and provided a substrate for coupling nDNA to polystyrene. This modified ELISA allowed the quantitation of antibodies to native DNA without the simultaneous binding of anti-histone antibodies.
我们评估了一种用于检测抗天然DNA（nDNA）抗体的酶联免疫吸附测定（ELISA）方法，该方法中使用鱼精蛋白将DNA连接到聚苯乙烯上。抗nDNA水平升高主要局限于系统性红斑狼疮（SLE）患者，并且在该组患者中，ELISA与硫酸铵测定法之间具有良好的相关性。然而，通常观察到大量背景免疫球蛋白与包被有鱼精蛋白的孔结合，因此有必要从每次抗DNA测定中减去这种活性。许多SLE血清中还含有抗组蛋白抗体，并且这种抗体活性与与鱼精蛋白的结合显示出显著相关性。相比之下，甲基化牛血清白蛋白（mBSA）不结合抗组蛋白抗体，并为将nDNA偶联到聚苯乙烯提供了底物。这种改良的ELISA方法能够定量抗天然DNA抗体，而不会同时结合抗组蛋白抗体。
Zotero 插件