Epigenomic Changes after Acupuncture Treatment in Patients Suffering from Burnout.

INTRODUCTION

The effects of acupuncture treatment in patients suffering from burnout may imply an epigenetic control mediated by DNA methylation changes. In this observational study, a genome-wide characterization of epigenetic changes in blood DNA, before and after acupuncture treatment, was performed in a cohort of 11 patients suffering from burnout.

METHODS

Burnout was assessed using the Maslach Burnout Inventory (MBI) and DNA was extracted from blood samples and analyzed by Illumina EPIC BeadChip.

RESULTS

Before acupuncture, all patients suffered of emotional exhaustion (EE) (MBI-EE score, 44 ± 6), 81% suffered of depersonalization (DP) (MBI-DP score, 16 ± 6), and 72% of low feelings of personal accomplishment (PA) (MBI-PA score, 29 ± 9). After acupuncture, all MBI dimensions improved significantly (EE, 16 ± 11 [p = 1.5 × 10-4]; DP, 4 ± 5 [p = 5.3 × 10-4]; and PA, 40 ± 6 [p = 4.1 × 10-3]). For each patient, both methylomes obtained before and after acupuncture co-clustered in the multidimensional scaling plot, indicating a high level of similarity. Genes corresponding to the 10 most differentially methylated CpGs showed enrichment in the brain dopaminergic signaling, steroid synthesis and in the insulin sensitivity pathways.

CONCLUSION

Acupuncture treatment was found to be highly effective on all burnout dimensions and the epigenetic targets identified were involved in some major disturbances of this syndrome.