通过傅里叶变换红外光谱（FTIR）和超高效液相色谱-四极杆/轨道阱质谱联用仪（UHPLC-Q/Orbitrap/MS）对五种非白色念珠菌抗生素耐药性的减弱、细胞毒性、抗炎作用及植物化学特征进行研究。

The attenuation of antibiotic resistant non-albicans species, cytotoxicity, anti-inflammatory effects and phytochemical profiles of five species by FTIR and UHPLC-Q/Orbitrap/MS.

作者信息

More Garland Kgosi, Chokwe Christinah Ramakwala, Meddows-Taylor Stephen

机构信息

College of Agriculture and Environmental Sciences Laboratories, University of South Africa, Florida, Johannesburg, 1710, South Africa.

Department of Chemistry, College of Science Engineering and Technology, University of South Africa, Florida, Johannesburg, 1710, South Africa.

出版信息

Heliyon. 2021 Nov 19;7(11):e08425. doi: 10.1016/j.heliyon.2021.e08425. eCollection 2021 Nov.

DOI:10.1016/j.heliyon.2021.e08425

PMID:34877423

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8632836/

Abstract

This work investigated the antifungal, cytotoxic and LPS-induced anti-inflammatory effects of five species (, , and ). The antifungal activity of the aqueous-methanolic extracts were performed using the broth dilution method against four non-albicans species (, and ). The cytotoxic and anti-inflammatory effects of the extracts were evaluated on African green monkey Vero kidney cells using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and the 2',7'-dichlorofluorescin diacetate (HDCF-DA) method. The fourier-transform infrared spectroscopy (FTIR) and Q Exactive plus orbitrap™ Ultra-high-performance liquid chromatography-mass spectrometer (UHPLC-MS) analysis was conducted to evaluate phytochemical constituents of the extracts. The plant extracts selected in this study displayed potency against the species tested, with MIC values ≤0.62 mg/mL for , and . A dose-dependent cell viability was observed on Vero cells with all extracts showing LC values >20 μg/mL. Extracts tested at 10 μg/mL elicited a significant decrease in lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) in Vero cells with , , and displaying inhibitory percentages of 35%, 32%, 55%, 52% and 49%, respectively. Characterisation of functional groups representing compounds in the extracts demonstrated the presence of different classes of compounds of the aliphatic, sugar and aromatic types. The Q Exactive plus orbitrap™ mass spectrometer enabled tentative identification of three major compounds in the extracts, including epigallocatechin, methyl gallate and quercetin amongst others. Based on the mass spectrometer results, it is postulated that quercetin found mostly in active extracts of , and may be responsible for the observed antifungal and anti-inflammatory activity. This data demonstrates that the species that were investigated could potentially be promising candidates for the management of fungal infections and related inflammation.

摘要

本研究调查了五种植物（、、和）的抗真菌、细胞毒性以及脂多糖诱导的抗炎作用。采用肉汤稀释法对四种非白色念珠菌（、和）进行了水-甲醇提取物的抗真菌活性测试。使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法和2',7'-二氯荧光素二乙酸酯（HDCF-DA）法，在非洲绿猴肾Vero细胞上评估了提取物的细胞毒性和抗炎作用。进行了傅里叶变换红外光谱（FTIR）和Q Exactive plus orbitrap™超高效液相色谱-质谱仪（UHPLC-MS）分析，以评估提取物的植物化学成分。本研究中选择的植物提取物对测试的菌株显示出效力，对于、和，其最低抑菌浓度（MIC）值≤0.62 mg/mL。在Vero细胞上观察到所有提取物的细胞活力呈剂量依赖性，LC值均>20 μg/mL。在10 μg/mL浓度下测试的提取物使Vero细胞中脂多糖（LPS）诱导的活性氧（ROS）显著降低，、、和的抑制率分别为35%、32%、55%、52%和49%。代表提取物中化合物的官能团表征表明存在脂肪族、糖类和芳香族等不同类别的化合物。Q Exactive plus orbitrap™质谱仪能够初步鉴定提取物中的三种主要化合物，包括表没食子儿茶素、没食子酸甲酯和槲皮素等。基于质谱仪结果推测，主要存在于、和活性提取物中的槲皮素可能是观察到的抗真菌和抗炎活性的原因。该数据表明，所研究的这几种植物可能是治疗真菌感染及相关炎症的有前景的候选植物。