McCall E, Rathbun R K, Riscoe M, Wilkinson B, Bagby G C
Exp Hematol. 1986 Sep;14(8):789-93.
Monocyte-derived recruiting activity (MRA) stimulates the release of granulopoietic colony-stimulating factors (CSF) by endothelial cells. We carried out studies designed to test the hypotheses that human placental conditioned medium (HPCM), a widely utilized source of CSA for in vitro studies, contains both MRA and CSA and that these molecules could be separated on the basis of their isoelectric points. In five separate studies, concentrated samples of HPCM were chromatofocused and fractions tested in bioassays for MRA and CSA. We found that HPCM contains both MRA and CSA, that the majority of the MRA eluted at a pH of from 7.6 to 8.2 and was 2000-fold purified. CSA eluted at pH 5.6 or below. No CSA was detectable in the major MRA peak. We conclude that HPCM contains heterogeneous granulopoietic activities, that the isoelectric points of MRA and CSA are substantially different and that chromatofocusing provides a rapid single-step method for separating these two distinct granulopoietic factors from complex conditioned media.
单核细胞衍生的募集活性（MRA）刺激内皮细胞释放粒细胞生成集落刺激因子（CSF）。我们开展了一些研究，旨在检验以下假设：人胎盘条件培养基（HPCM）作为体外研究中广泛使用的CSF来源，同时含有MRA和CSF，并且这些分子可以根据其等电点进行分离。在五项独立研究中，对浓缩的HPCM样本进行了层析聚焦，并对各组分进行了MRA和CSF生物测定。我们发现HPCM同时含有MRA和CSF，大部分MRA在pH值7.6至8.2时洗脱，纯化倍数为2000倍。CSF在pH 5.6或更低时洗脱。在主要的MRA峰中未检测到CSF。我们得出结论，HPCM含有异质性粒细胞生成活性，MRA和CSF的等电点有显著差异，层析聚焦为从复杂的条件培养基中分离这两种不同的粒细胞生成因子提供了一种快速的单步方法。
