Aisenberg A C, Wilkes B M, Jacobson J O
Department of Medicine, Massachusetts General Hospital, Boston 02114.
J Clin Invest. 1987 Oct;80(4):1209-14. doi: 10.1172/JCI113182.
We determined the configuration of the genes for the beta (T beta) and gamma (T gamma) chains of the T cell receptor in DNA from 100 consecutive cases of B cell lymphoma and B cell chronic lymphocytic leukemia (B-CLL), and compared the findings with those in 18 T cell neoplasms. In 7 of the 100 B cell specimens, a single nongermline band was detected after digestion with the restriction enzyme BamHI, but the rearrangement could be confirmed with a second restriction enzyme in only two. The B cell fragments were small in size and of limited size diversity when compared with the T cell cases, and germline bands of equal intensity were present. A rearrangement of the T gamma gene was never seen in a B cell sample. In contrast, T cell specimens usually rearranged both alleles of T beta (15 of 18), the rearrangement could be confirmed with a second restriction enzyme (17 of 18), both alleles of the first constant region gene segment of T beta always underwent either rearrangement or deletion, and the T gamma gene was also rearranged or deleted (17 of 18). We conclude that ordered rearrangement of the T cell receptor is a rare event in B cell lymphoma and B-CLL. T cell receptor gene studies allow B and T cell lymphomas to be distinguished from each other and from common acute lymphoblastic leukemia antigen-positive non-T, non-B acute lymphoblastic leukemia.
我们测定了100例连续的B细胞淋巴瘤和B细胞慢性淋巴细胞白血病（B-CLL）患者DNA中T细胞受体β链（Tβ）和γ链（Tγ）基因的构型，并将结果与18例T细胞肿瘤的结果进行比较。在100个B细胞标本中的7个中，用限制性内切酶BamHI消化后检测到一条单一的非种系条带，但只有两个能用第二种限制性内切酶证实重排。与T细胞病例相比，B细胞片段尺寸小且大小多样性有限，并且存在强度相等的种系条带。在B细胞样本中从未见过Tγ基因重排。相反，T细胞标本中Tβ的两个等位基因通常都发生重排（18例中的15例），能用第二种限制性内切酶证实重排（18例中的17例），Tβ第一个恒定区基因片段的两个等位基因总是发生重排或缺失，并且Tγ基因也发生重排或缺失（18例中的17例）。我们得出结论，T细胞受体的有序重排在B细胞淋巴瘤和B-CLL中是罕见事件。T细胞受体基因研究可使B细胞和T细胞淋巴瘤相互区分，并与常见的急性淋巴细胞白血病抗原阳性的非T、非B急性淋巴细胞白血病相区分。
