Han Lingjuan, Li Yixuan, Ma Zheng, Li Bin, Liang Yinping, Gao Peng, Zhao Xiang
College of Grassland Science, Shanxi Agricultural University, Jinzhong 030801, China.
College of Horticulture, Shanxi Agricultural University, Jinzhong 030801, China.
Plants (Basel). 2025 Sep 2;14(17):2744. doi: 10.3390/plants14172744.
Soil salinization critically threatens global agricultural productivity by impairing plant growth and soil fertility. This study investigated the potential of a consortium, comprising DP25, DP28, and DP29, to enhance the saline-alkali tolerance of alfalfa and improve soil properties. The experiments comprised five germination treatments (saline control, each strain alone, consortium) and three pot treatments (non-saline control, saline control, consortium). Under saline-alkali stress, co-inoculation with the consortium significantly ( < 0.05) increased alfalfa seed germination rates, emergence rates, and biomass (shoot and root dry weight), while promoting root development. Physiological analyses revealed that the bacterial consortium mitigated stress-induced damage by enhancing photosynthetic efficiency, chlorophyll content, and antioxidant enzyme activities (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT)), while decreasing malondialdehyde (MDA) levels. Moreover, the inoculant improved osmoprotectant accumulation (soluble sugars, soluble proteins, and proline) and modulated soil properties by reducing pH and electrical conductivity (EC), while elevating nutrient availability and soil enzyme activities. Correlation and principal component analyses (PCA) confirmed strong associations among improved plant growth, physiological traits, and soil health. These findings demonstrate that the bacterial consortium effectively alleviates saline-alkali stress in alfalfa by improving soil health, offering a sustainable strategy for ecological restoration and improving agricultural productivity in saline-alkali regions.
土壤盐渍化通过损害植物生长和土壤肥力严重威胁全球农业生产力。本研究调查了由DP25、DP28和DP29组成的联合体提高苜蓿耐盐碱能力和改善土壤性质的潜力。实验包括五种发芽处理（盐对照、各菌株单独处理、联合体处理）和三种盆栽处理（非盐对照、盐对照、联合体处理）。在盐碱胁迫下，与联合体共同接种显著（<0.05）提高了苜蓿种子发芽率、出苗率和生物量（地上部和根部干重），同时促进了根系发育。生理分析表明，细菌联合体通过提高光合效率、叶绿素含量和抗氧化酶活性（超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT））减轻了胁迫诱导的损伤，同时降低了丙二醛（MDA）水平。此外，接种剂通过降低pH值和电导率（EC）改善了渗透保护剂的积累（可溶性糖、可溶性蛋白和脯氨酸）并调节了土壤性质，同时提高了养分有效性和土壤酶活性。相关性分析和主成分分析（PCA）证实了植物生长改善、生理性状和土壤健康之间的强关联。这些发现表明，细菌联合体通过改善土壤健康有效缓解了苜蓿的盐碱胁迫，为盐碱地区的生态恢复和提高农业生产力提供了一种可持续策略。
