College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
Carbohydr Polym. 2022 Feb 1;277:118782. doi: 10.1016/j.carbpol.2021.118782. Epub 2021 Oct 19.
This work aims to fabricate multifunctional hemostatic sponges (C-ODs). Porous C-ODs were first constructed by using capric acid-modified chitosan (CSCA) and oxidized dextrans (ODs) with different oxidation degrees. Batches of experiments showed that (i) CSCA (33.39% of grafting degree), ODs, and C-ODs (100-200 μm in pore size) were synthesized, evidenced by FT-IR, H NMR, elemental analysis, hydroxylamine hydrochloride titration, and SEM results; (ii) among C-ODs, C-OD had appropriate porosity (85.0%), swelling (20 times its dry weight), absorption, water retention, water vapor transmission, and mechanical properties; (iii) C-OD possessed low toxicity (relative cell viability > 86%), low hemolysis rate (0.65%), suitable tissue adhesion (4.74 kPa), and strong antibacterial efficacy (five strains); and (iv) C-OD's dynamic blood clotting was within 30 s. In three animal injury models, C-OD's hemostasis time and blood loss were fairly lower than commercial gelatin sponge. Totally, C-OD might serve as an ideal hemostatic dressing.
这项工作旨在制备多功能止血海绵（C-ODs）。首先通过使用己酸改性壳聚糖（CSCA）和不同氧化度的氧化葡聚糖（ODs）构建多孔 C-ODs。一系列实验表明：（i）合成了 CSCA（接枝度为 33.39%）、ODs 和 C-ODs（孔径为 100-200μm），这可以通过 FT-IR、H NMR、元素分析、盐酸羟胺滴定和 SEM 结果证明；（ii）在 C-ODs 中，C-OD 具有适当的孔隙率（85.0%）、溶胀性（干重的 20 倍）、吸收性、保水性、水蒸气透过性和机械性能；（iii）C-OD 具有低毒性（相对细胞活力>86%）、低溶血率（0.65%）、适当的组织粘附力（4.74kPa）和强大的抗菌功效（五种菌株）；（iv）C-OD 的动态凝血时间在 30s 内。在三种动物损伤模型中，C-OD 的止血时间和失血量明显低于商业明胶海绵。总的来说，C-OD 可能是一种理想的止血敷料。
