College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao 266003, PR China.
National Engineering Technology Research Center for Marine Drugs, Marine Biomedical Research Institute of Qingdao, Ocean University of China, Qingdao 266003, PR China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 1):124669. doi: 10.1016/j.ijbiomac.2023.124669. Epub 2023 May 6.
In this study, porous hemostatic sponges (CGS1, CGS2 and CGS3) with proper absorption (38-43×) and air permeability (2214 g/m·day) were prepared from l-glutamine-modified chitosan (CG), tannic acid-modified gelatin (GTA), and oxidized dextran (ODEX) by Schiff base crosslinking reaction. Among them, CGS2 was proved to have high porosity (88.98 %), durable water retention (>6 h), strong antibacterial activity, proper mechanical quality, and suitable tissue adhesion. In addition, CGS2 had good biocompatibility, mainly manifested in low hemolysis rate (<0.4 %), low cytotoxicity (relative cell activity>90 %), and good biodegradability in vitro. The hemostatic time and blood loss in CGS2 group were much lower than those in commercial gelatin sponge group in three animal injury models. Moreover, the activated partial thromboplastin time (APTT) and the prothrombin time (PT) results indicated that CGS2 promoted coagulation by activating the endogenous coagulation pathway. These results suggested that CGS2 had great potential for rapid hemostasis and avoidance of wound infection.
在这项研究中，通过席夫碱交联反应，从 l-谷氨酰胺改性壳聚糖（CG）、单宁酸改性明胶（GTA）和氧化葡聚糖（ODEX）制备了具有适当吸收（38-43×）和透气性（2214 g/m·天）的多孔止血海绵（CGS1、CGS2 和 CGS3）。其中，CGS2 被证明具有高孔隙率（88.98%）、持久的保水能力（>6 小时）、强大的抗菌活性、适当的机械质量和合适的组织粘附性。此外，CGS2 具有良好的生物相容性，主要表现为低溶血率（<0.4%）、低细胞毒性（相对细胞活力>90%）和体外良好的可生物降解性。在三种动物损伤模型中，CGS2 组的止血时间和失血量明显低于商业明胶海绵组。此外，活化部分凝血活酶时间（APTT）和凝血酶原时间（PT）结果表明，CGS2 通过激活内源性凝血途径促进凝血。这些结果表明，CGS2 具有快速止血和避免伤口感染的巨大潜力。
