Du Xinchen, Zhang Tongxing, Liu Yadong, Li Tong, Yang Jiuxia, Li Xuelei, Wang Lianyong
Department of Chemical and Environmental Engineering, Hetao College, Bayannaoer, Inner Mongoli, 015000, China.
Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin, 300071, China.
Mater Today Bio. 2024 Mar 23;26:101031. doi: 10.1016/j.mtbio.2024.101031. eCollection 2024 Jun.
Developing a self-elastic sponge integrating active and passive hemostatic mechanisms for the effective management of uncontrolled coagulopathic hemorrhage remains a challenge. We here developed a chitosan-based sponge by integrating freeze-drying, chemical decoration of alkyl chains and phosphate groups, and physical loading of thrombin. The sponge exhibited high mechanical strength, self-elasticity, and rapid shape recovery. The sponge facilitated blood cell adhesion, aggregation, and activation through hydrophobic and electrostatic interactions, as well as accelerated blood clotting. The sponge exhibited higher efficacy than commercial gauze and gelatin sponge in managing uncontrolled hemorrhage from heparinized rat tail amputation, liver superficial injury, and liver perforating wound models. In addition, the sponge exhibited favorable biodegradability and biocompatibility. These findings revealed that the developed sponge holds great potential as a novel hemostat for effectively managing uncontrolled coagulopathic hemorrhage from superficial and perforating wounds.
开发一种整合主动和被动止血机制的自弹性海绵，以有效管理失控的凝血病性出血仍然是一项挑战。我们在此通过整合冷冻干燥、烷基链和磷酸基团的化学修饰以及凝血酶的物理负载，开发了一种基于壳聚糖的海绵。该海绵具有高机械强度、自弹性和快速形状恢复能力。海绵通过疏水和静电相互作用促进血细胞粘附、聚集和活化，并加速血液凝固。在处理肝素化大鼠尾巴截肢、肝脏浅表损伤和肝脏穿孔伤口模型的失控出血方面，该海绵比商用纱布和明胶海绵表现出更高的疗效。此外，该海绵具有良好的生物降解性和生物相容性。这些发现表明，所开发的海绵作为一种新型止血剂，在有效管理浅表和穿孔伤口的失控凝血病性出血方面具有巨大潜力。
