Huang Ailin, Wu Tao, Wu Xiuyun, Zhang Biao, Shen Yuanyuan, Wang Suying, Song Wenjun, Ruan Haihua
Tianjin Key Laboratory of Food Science and Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
Front Microbiol. 2021 Nov 25;12:752791. doi: 10.3389/fmicb.2021.752791. eCollection 2021.
The parasitoid fungus , whose fruiting bodies are known in China as "chan hua," literally "cicada flower," has been used as a traditional Chinese medicinal ingredient for centuries. However, systematic disclosure of the vital factors responsible for the formation of wild cicada flower is limited. Here, we determined the physicochemical properties of soil and simultaneously analyzed the diversities and the structures of microbial community inhabiting the coremia, sclerotia, and soil around wild cicada flowers through high-throughput sequencing. Our results indicated that cicada flower more preferentially occurred in acidic soil (pH 5.9) with abundant moisture content (MC), total nitrogen (TN), and organic matter (OM). The dominant fungal genera in soil mainly included , f__Clavariaceae_Unclassified, , f__Chaetomiaceae_Unclassified, , f__Sordariaceae_Unclassified, and . Among them, was the only fungus that was massively detected in both the coremia and sclerotia with abundance of 83.5 and 53.6%, respectively. Based on this, a strain named AH10-4 with excellent adenosine- and -(2-hydroxyethyl)-adenosine (HEA)-producing capability was successfully isolated. However, to the aspect of bacteria, , , , f__Xanthobacteraceae_Unclassified, and were the dominant genera in soil. , f__Enterobacteriaceae_Unclassified, , , , , and were the dominant genera in the coremia and sclerotia. Notably, was the shared bacteria among them with high abundance of 3.1, 11.4, and 5.2% in the sclerotia, coremia, and soil, respectively. However, the possible role of these bacteria to the occurrence of cicada flower has been unclear to our knowledge. By analyzing the correlation between physicochemical properties and microbial community of soil, we found that MC, Fe, and Zn were significantly negatively correlated with soil and that Cu was significantly negatively correlated with most dominant soil bacterial genera. But Mg was significantly positively correlated with most dominant taxa. This study provides new insight into the formation mechanisms of cicada flower and may contribute to the large-scale cultivation of cicada flowers.
这种寄生真菌，其子实体在中国被称为“蝉花”，字面意思是“知了花”，几个世纪以来一直被用作传统中药成分。然而，关于野生蝉花形成的关键因素的系统披露有限。在此，我们测定了土壤的理化性质，并通过高通量测序同时分析了野生蝉花的菌索、菌核及周围土壤中微生物群落的多样性和结构。我们的结果表明，蝉花更倾向于出现在酸性土壤（pH 5.9）中，这种土壤具有丰富的含水量（MC）、总氮（TN）和有机质（OM）。土壤中的优势真菌属主要包括、未分类的棒瑚菌科、、未分类的毛壳菌科、、未分类的粪壳菌科和。其中，是唯一在菌索和菌核中均大量检测到的真菌，其丰度分别为83.5%和53.6%。基于此，成功分离出一株具有优异产腺苷和2-(2-羟乙基)-腺苷（HEA）能力的菌株AH10-4。然而，在细菌方面，、、、未分类的黄色杆菌科和是土壤中的优势属。、未分类的肠杆菌科、、、、和是菌索和菌核中的优势属。值得注意的是，是它们之间共有的细菌，在菌核、菌索和土壤中的丰度分别高达3.1%、11.4%和5.2%。然而，据我们所知，这些细菌对蝉花出现的可能作用尚不清楚。通过分析土壤理化性质与微生物群落之间的相关性，我们发现MC、Fe和Zn与土壤真菌显著负相关，而Cu与大多数优势土壤细菌属显著负相关。但Mg与大多数优势分类群显著正相关。本研究为蝉花的形成机制提供了新的见解，并可能有助于蝉花的大规模栽培。
