Shao Jun-Li, Lai Bei, Jiang Wei, Wang Jia-Ting, Hong Yue-Hui, Chen Fu-Bin, Tan Shao-Qing, Guo Lian-Xian
Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan 523808, China.
Department of Basic Medicine, Guangdong Jiangmen Chinese Medical College, Jiangmen 529000, China.
Microorganisms. 2019 Aug 22;7(9):284. doi: 10.3390/microorganisms7090284.
Chinese is a well-known medicinal larva-fungus symbiote distributed in the Qinghai-Tibetan Plateau and adjacent areas. Previous studies have involved its artificial cultivation but commercial cultivation is difficult to perform because the crucial factors triggering the occurrence of Chinese are not quite clear. The occurrence of Chinese is greatly affected by the soil environment, including the soil's physicochemical and microecological properties. In this study, the effects of these soil properties on the occurrence of Chinese were investigated. The results show that the physicochemical properties, including easily oxidizable organic carbon (EOC), soil organic carbon (SOC), humic acid carbon (HAC), humin carbon (HMC), and pH, might be negatively related to the occurrence of Chinese , and soil water content (SWC) might be positively related. Several soil physicochemical parameters (pH, SOC, HMC, HAC, available potassium (APO), available phosphorus (APH), microbial biomass carbon (MBC), and the ratio of NH to NO (NH/NO)) and microbial properties interact and mix together, which might affect the occurrence of Chinese . Soil microbial community structure was also a possible factor, and a low level of bacterial and fungal diversity was suitable for the occurrence of Chinese . The intra-kingdom network revealed that a closer correlation of the bacterial community might help the occurrence of Chinese , while a closer correlation of the fungal community might suppress it. The inter-kingdom network revealed that the occurrence rate of Chinese might be negatively correlated with the stability of the correlation state of the soil habitat. In conclusion, this study shows that soil physicochemical properties and microbial communities could be greatly related with the occurrence of Chinese . In addition, soil physicochemical properties, the level of bacterial and fungal diversity, and correlations of bacterial and fungal communities should be controlled to a certain level to increase the production of Chinese in artificial cultivation.
冬虫夏草是一种分布于青藏高原及其周边地区的著名药用幼虫-真菌共生体。以往的研究涉及到其人工栽培，但由于引发冬虫夏草发生的关键因素尚不完全清楚，商业栽培难以进行。冬虫夏草的发生受到土壤环境的极大影响，包括土壤的理化性质和微生态性质。在本研究中，研究了这些土壤性质对冬虫夏草发生的影响。结果表明，包括易氧化有机碳（EOC）、土壤有机碳（SOC）、腐殖酸碳（HAC）、胡敏素碳（HMC）和pH值在内的理化性质可能与冬虫夏草的发生呈负相关，而土壤含水量（SWC）可能呈正相关。几个土壤理化参数（pH值、SOC、HMC、HAC、有效钾（APO）、有效磷（APH）、微生物生物量碳（MBC）以及NH与NO的比值（NH/NO））和微生物性质相互作用并混合在一起，这可能会影响冬虫夏草的发生。土壤微生物群落结构也是一个可能的因素，低水平的细菌和真菌多样性适合冬虫夏草的发生。界内网络显示，细菌群落的更紧密相关性可能有助于冬虫夏草的发生，而真菌群落的更紧密相关性可能会抑制它。界间网络显示，冬虫夏草的发生率可能与土壤栖息地相关状态的稳定性呈负相关。总之，本研究表明土壤理化性质和微生物群落可能与冬虫夏草的发生密切相关。此外，应将土壤理化性质、细菌和真菌多样性水平以及细菌和真菌群落的相关性控制在一定水平，以提高人工栽培中冬虫夏草的产量。
