Rasheed S, Gottlieb A A, Garry R F
Virology. 1986 Oct 30;154(2):395-400. doi: 10.1016/0042-6822(86)90465-4.
Extensive cell killing and cytopathology were observed within 24 hr after exposure of a clonal cell line of human T-4 lymphocytes (RH9) to culture supernatants containing human immunodeficiency virus (HIV). Ultraviolet-irradiated HIV-containing culture fluids were also capable of killing RH9 cells and of inducing specific cytopathic effects which were indistinguishable from those induced by unirradiated virus-containing preparations. The uv-irradiated HIV was incapable of forming proviral DNA using the endogenous virion genomic RNA as a template. The RH9 cells persistently infected with HIV did not release soluble cytotoxic factors to account for the cell killing observed when culture supernatants were added to uninfected RH9 cells. The fraction involved in cell killing had the hydrodynamic properties of a retrovirus. These results suggest that a virion component is responsible for cell killing by HIV.
在将人T - 4淋巴细胞克隆细胞系（RH9）暴露于含人类免疫缺陷病毒（HIV）的培养上清液后24小时内，观察到广泛的细胞杀伤和细胞病理学变化。经紫外线照射的含HIV培养液也能够杀死RH9细胞，并诱导出与未照射的含病毒制剂所诱导的特异性细胞病变效应无法区分的效应。经紫外线照射的HIV无法以内源性病毒粒子基因组RNA为模板形成前病毒DNA。持续感染HIV的RH9细胞不会释放可溶性细胞毒性因子来解释当将培养上清液添加到未感染的RH9细胞中时所观察到的细胞杀伤现象。参与细胞杀伤的部分具有逆转录病毒的流体动力学特性。这些结果表明，病毒粒子成分是导致HIV细胞杀伤的原因。
