Jin Xinxin, Yuan Boyu, Liu Mingming, Zhu Mingqiang, Zhang Xue, Xie Gaijie, Wu Wenxiang, Wang Zifan, Xu Haidong, Lv Yantao, Huang Yanhua, Wang Wei
College of Animal Science & Technology, Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou, China.
College of Veterinary Medicine, Jilin University, Changchun, China.
Front Vet Sci. 2021 Nov 25;8:746224. doi: 10.3389/fvets.2021.746224. eCollection 2021.
A high-quality protein substitute, (black soldier fly) larvae powder, is rich in protein and often used in animal feed. This study aimed to investigate the feasibility and optimal ratio of replacing fish meal with larvae in weaned piglets and to demonstrate the effects on piglets' growth performance, intestinal microflora and immune performance. Forty-eight female weaned piglets were randomly classified into three groups. Each group consisted of eight pens (replicates), with two piglets per pen. Three groups containing different proportions of larvae (0, 4, and 8%) were referred to as C, HI4, and HI8. We first designed a 28-day feeding experiment to detect growth performance; after that, the piglets were induced with oral gavage of enterotoxigenic K88 (ETEC K88) and recording diarrhea on day 29 of the experiment. Samples were taken on the 32nd day to detect the effect of larvae on the immune performance of the weaned piglets. larvae replacement did not cause any obvious change in the growth performance nether in HI4 nor in HI8 of weaned piglets with 28 d feeding stage. larvae could improve the intestinal health of weaned piglets by increasing the content of and reducing the content of . Compared with C+K88 group, the diarrhea rate was attenuated for the supplemented group. The integrity of ileum villi in HI4+K88 and HI8+K88 groups was better than that in C+K88 group, and the villi in C+K88 group were severely damaged. The expression of and in the intestinal mucosa of the HI4+K88 group and HI8+K88 group were significantly increased ( < 0.05), and the expression of -α was significantly decreased ( < 0.05) compared with the C+K88 group. The results of immunoblotting also validated that the same ETEC K88 treatment of weaned piglets enhanced the expression of tight junction protein in the intestinal mucosa of the addition group. ETEC-induced diarrhea will be reduced by the diet of weaned piglets containing larvae, ameliorating the immune performance of piglets. Our results indicates that the optimal dosage of replacement in weaned piglets is 4%.
一种优质蛋白质替代品，（黑水虻）幼虫粉，富含蛋白质，常用于动物饲料。本研究旨在探讨断奶仔猪中用幼虫替代鱼粉的可行性和最佳比例，并证明其对仔猪生长性能、肠道微生物群和免疫性能的影响。48只雌性断奶仔猪被随机分为三组。每组由8个栏（重复）组成，每栏有2只仔猪。含有不同比例幼虫（0%、4%和8%）的三组分别称为C组、HI4组和HI8组。我们首先设计了一个28天的饲养实验来检测生长性能；之后，在实验的第29天对仔猪进行口服产肠毒素大肠杆菌K88（ETEC K88）诱导并记录腹泻情况。在第32天采集样本以检测幼虫对断奶仔猪免疫性能的影响。在28天的饲养阶段，幼虫替代在HI4组和HI8组的断奶仔猪生长性能方面未引起任何明显变化。幼虫可通过增加双歧杆菌含量和降低大肠杆菌含量来改善断奶仔猪的肠道健康。与C + K88组相比，添加幼虫的组腹泻率降低。HI4 + K88组和HI8 + K88组回肠绒毛的完整性优于C + K88组，C + K88组的绒毛严重受损。与C + K88组相比，HI...（此处原文似乎有缺失）4 + K88组和HI8 + K88组肠黏膜中ZO-1和Occludin的表达显著增加（P < 0.05），而Claudin-α的表达显著降低（P < 0.05）。免疫印迹结果也证实，相同的ETEC K88处理断奶仔猪可增强添加幼虫组肠黏膜中紧密连接蛋白的表达。含有幼虫的断奶仔猪日粮可减少ETEC诱导的腹泻，改善仔猪的免疫性能。我们的结果表明，断奶仔猪中幼虫替代的最佳剂量为4%。
