Tianjin Institute for Drug Control (TIDC), Tianjin 300070, PR China.
National Institutes for Food and Drug Control, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005158.
A Gram-stain-positive, facultatively anaerobic, non-motile, endospore-forming and rod-shaped bacterium, occurring singly or in pairs, designated TB2019, was isolated from environmental monitoring samples of corridor air collected at the Tianjin Institute for Drug Control, Tianjin Province (PR China). The isolate was able to grow at 15-40 °C (optimum growth at 37 °C), pH 6.0-8.0 (pH 7.0) and in the presence of 0-2% (w/v) NaCl (0% NaCl). Comparison of 16S rRNA gene sequences indicated that TB2019 was most closely related to CGMCC 1.11012 (98.63%), Q4-3 (98.19%), DSM 13188 (97.55%), P26E (97.33%) and DSM 15391 (97.19%). The digital DNA-DNA hybridization and the average nucleotide identity values between TB2019 and the five type strains mentioned above ranged from 20.7 to 25.0% and 75.2 to 81.3%, respectively, and the genomic DNA G+C content was 49.52 mol%. The diagnostic cell-wall sugar was ribose, and the diagnostic amino acid was -diaminopimelic acid. The polar lipids of TB2019 included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unidentified aminophospholipids and one unidentified phospholipid. MK-7 was the predominant menaquinone, and anteiso-C (30.6%) was the major fatty acid. Based on the polyphasic taxonomic data, strain TB2019 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TB2019 (=CICC 25065=JCM 34610).
一株革兰氏阳性、兼性厌氧、无动力、产芽孢的杆状细菌,以单生或成对形式出现,从中国天津药物研究院环境监测的走廊空气中采集的样本中分离得到,命名为 TB2019。该分离株能够在 15-40°C(最适生长温度为 37°C)、pH 6.0-8.0(pH 7.0)和 0-2%(w/v)NaCl(无 NaCl)的条件下生长。16S rRNA 基因序列比较表明,TB2019 与 CGMCC 1.11012(98.63%)、Q4-3(98.19%)、DSM 13188(97.55%)、P26E(97.33%)和 DSM 15391(97.19%)最为密切相关。数字 DNA-DNA 杂交和 TB2019 与上述 5 个模式菌株之间的平均核苷酸同一性值分别为 20.7-25.0%和 75.2-81.3%,基因组 DNA G+C 含量为 49.52mol%。细胞壁糖为核糖,诊断性氨基酸为 -二氨基庚二酸。TB2019 的极性脂包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、三种未鉴定的氨基磷脂和一种未鉴定的磷脂。MK-7 是主要的menaquinone,anteiso-C(30.6%)是主要的脂肪酸。基于多相分类学数据,菌株 TB2019 代表了一个新的属种,提议命名为 sp. nov.。模式菌株为 TB2019(=CICC 25065=JCM 34610)。