Department of Microbiology, Chung-Ang University College of Medicine, Seoul 06974, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Dec;71(12). doi: 10.1099/ijsem.0.005159.
A novel Gram-negative, aerobic, non-motile, rod-shaped, bacterial strain (CAU 1598) was isolated from marine sand. Strain CAU 1598 grew well at 30 °C, pH 6.5-7.0 and with 3 % NaCl (w/v). Phylogeny results based on 16S rRNA gene sequencing indicated that the identified strain had the highest similarity (94.3%) to , indicating that strain CAU 1598 belongs to the family . Further, the fatty acid profile of the strain was primarily composed of C iso-C, iso-C, summed feature 3 (consisting of C 7/iso-C 2-OH) and summed feature 9 (consisting of iso-C 9 and/or C 10-methyl), with ubiquinone-8 as the major isoprenoid quinone. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphoglycolipid, an unidentified aminolipid and an unidentified lipid. The G+C content of the bacterial genome was 62.6 mol% and its 5.4 Mb length encompassed 144 contigs and 4236 protein-coding genes. These phenotypic, chemotaxonomic and phylogenetic data indicate that CAU 1598 belongs to a new genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is CAU 1598 (=KCTC 82406=MCCC 1K05673).
从海洋砂中分离到一株革兰氏阴性、需氧、不运动、杆状的细菌菌株（CAU 1598）。菌株 CAU 1598 在 30°C、pH6.5-7.0 和 3%NaCl（w/v）条件下生长良好。基于 16S rRNA 基因测序的系统发育结果表明，该鉴定菌株与 具有最高的相似性（94.3%），表明菌株 CAU 1598 属于 科。此外，该菌株的脂肪酸图谱主要由 C iso-C、iso-C、summed feature 3（由 C 7/iso-C 2-OH 组成）和 summed feature 9（由 iso-C 9 和/或 C 10-methyl 组成）组成，主要类异戊二烯醌为 ubiquinone-8。极性脂谱包括二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷酯糖脂、一种未鉴定的胺脂和一种未鉴定的脂。细菌基因组的 G+C 含量为 62.6mol%，其 5.4Mb 长度包含 144 个连续序列和 4236 个蛋白质编码基因。这些表型、化学分类和系统发育数据表明，CAU 1598 属于一个新属和新种，建议采用新属名 gen. nov.，新种名 sp. nov.。模式菌株为 CAU 1598（=KCTC 82406=MCCC 1K05673）。
