Prevention of glucose-induced cataract by prepared by (paste of Linn).

BACKGROUND

Cataract is a major cause of blindness worldwide. Researchers received much attention from the traditional systems such as Ayurveda for the solutions of cataract through antioxidant activities apart from the surgical extraction.

AIMS

A To study, the anti-cataract activity of (VK) prepared with ( Linn) (paste) in (fat) of the domestic fowl () on glucose-induced cataract in ovine (sheep) lenses.

MATERIALS AND METHODS

Artificial aqueous humor with 55 mM glucose was used to induce cataract in sheep eye lenses. Treatment was given with cow ghee (CG), plain fat, , and Vitamin E to the same media and lenses were incubated at the room temperature for 72 h. Biochemical parameters studied in the lens were total proteins, malondialdehyde (MDA), Na K ATPase activity and electrolytes (Na and K). Photographic evaluation was also done.

RESULTS

The complete opacification induced by the glucose in ovine lens was observed in 72 h. Cataractous lenses showed significant increase in Na, MDA level and significant decrease in Na K ATPase activity and total protein content. Lenses treated with showed non-significant increase of total protein content and decreased MDA level and prevented formation and progress of cataract by glucose, as evidenced by photographic evaluation. Glucose-induced biochemical changes were found to be reversed in statistically significant manner in CG and Vitamin E treated lenses.

CONCLUSION

The anti-cataract activity of and CG may be because of the antioxidant and free radical scavenging activity. Further and studies in various experimental models are required to validate their anti-cataract activity.