Chou H S, Behlke M A, Godambe S A, Russell J H, Brooks C G, Loh D Y
EMBO J. 1986 Sep;5(9):2149-55. doi: 10.1002/j.1460-2075.1986.tb04478.x.
Both cDNA and genomic clones of the T cell receptor (TCR) alpha- and beta-chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non-functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six-member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7-14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general.
对同种异体反应性细胞毒性T淋巴细胞（CTL）克隆F3的T细胞受体（TCR）α链和β链基因的cDNA和基因组克隆进行了检测。在α和β基因座均发现了两个不同的重排事件，一个是功能性的，一个是非功能性的。因此，只能确定一个单一的功能性TCRαβ异二聚体，这与TCR基因中的等位基因排斥一致。F3使用的Vα基因是一个由六个成员组成的Vα亚家族的一部分。分离出包含该亚家族每个成员的基因组克隆，并确定了Vα核苷酸序列。这六个基因中有五个是功能性的；这些基因在氨基酸水平上彼此相差7%-14%。与一般Vα基因之间的可变模式相反，在这个亚家族中定义了一个单一的显性高变区。
