Rasouli Latifeh, Aryaeian Naheed, Gorjian Mehran, Nourbakhsh Mitra, Amiri Fatemehsadat
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, International Campus, Tehran, Iran.
Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
J Educ Health Promot. 2021 Oct 29;10:376. doi: 10.4103/jehp.jehp_1456_20. eCollection 2021.
Colorectal cancer is the third leading to death type of cancer in the world. The therapeutic guideline varied between different methods. As the main therapeutic guideline is chemotherapy, recent studies had shown utilization of natural products in combination with conventional medication, elevate the efficiency of chemotherapeutic methods. Kombucha is a traditional beverage obtained from the fermentation of green tea as a rich source of flavonoid medicinal plant. This study aimed to evaluate the natural potential of combination therapy of this natural product with doxorubicin as a chemotherapeutic agent.
The study was performed as evaluation of biological activity of kombucha on HCT-116 cell line (human colon cancer cell line). The cytotoxic effect of different kombucha beverages (fermented green tea) in comparison with green tea extract was evaluated by dimethylthiazolyl tetrazolium bromide (MTT) assay. In the next step, anticancer activity of doxorubicin as a general guideline chemotherapeutic agent in combination with kombucha was evaluated by cell cycle analysis and apoptosis assay flow cytometry. Apoptotic genes expression pattern was determined using real-time polymerase chain reaction. The experiments were designed in three independent replications and statistically analyzed using SPSS software.
The results show that kombucha compared with the green tea extract caused more (1.2 fold) early apoptosis induction and G0/G1 phase arrest. Moreover, kombucha increased the expression levels of p21, p53, and B-cell leukemia/lymphoma 2 (Bcl-2)-associated X protein genes (2, 2.5, and 1.5 fold, respectively) while it decreased Bcl-2 gene expression level (5-8 fold) compared with doxorubicin alone. Combination of kombucha with doxorubicin shows 2-fold increased G0/G1 phase compared with the doxorubicin treatment.
This result indicated that kombucha caused boosted anticancer activity of doxorubicin agent. These findings suggest that kombucha may be has an assistor and useful role in colorectal cancer treatment align with chemotherapy.
结直肠癌是全球第三大致死性癌症类型。不同治疗方法的治疗指南各不相同。由于主要治疗指南是化疗，最近的研究表明，天然产物与传统药物联合使用可提高化疗方法的效率。康普茶是一种通过绿茶发酵获得的传统饮品，是类黄酮药用植物的丰富来源。本研究旨在评估这种天然产物与化疗药物阿霉素联合治疗的天然潜力。
本研究通过评估康普茶对HCT-116细胞系（人结肠癌细胞系）的生物活性来进行。通过噻唑蓝（MTT）法评估不同康普茶饮品（发酵绿茶）与绿茶提取物相比的细胞毒性作用。下一步，通过细胞周期分析和凋亡检测流式细胞术评估阿霉素作为一般化疗指南药物与康普茶联合使用的抗癌活性。使用实时聚合酶链反应确定凋亡基因的表达模式。实验设计为三个独立重复，并使用SPSS软件进行统计分析。
结果表明，与绿茶提取物相比，康普茶导致更多（1.2倍）的早期凋亡诱导和G0/G1期阻滞。此外，与单独使用阿霉素相比，康普茶使p21、p53和B细胞淋巴瘤/白血病-2（Bcl-2）相关X蛋白基因的表达水平分别增加了2倍、2.5倍和1.5倍，同时降低了Bcl-2基因表达水平（5-8倍）。与阿霉素治疗相比，康普茶与阿霉素联合使用使G0/G1期增加了2倍。
该结果表明康普茶增强了阿霉素的抗癌活性。这些发现表明，康普茶在结直肠癌化疗治疗中可能具有辅助和有益作用。
