壳聚糖/海藻酸钠修饰提取液的银纳米粒子的绿色合成：对致病菌的杀菌活性和对正常细胞系的细胞毒性分析。

Green Synthesis of Silver Nanoparticles using Extract Modified by Chitosan/Alginate: Bactericidal Activity against Pathogenic Bacteria and Cytotoxicity Analysis in Normal Cell Line.

机构信息

Department of Microbiology, School of Basic Science, Islamic Azad University, Neyshabur Branch, Neyshabur, Iran.

Department of Food Science and Technology, Neyshabur University of Medical Sciences, Neyshabur, Iran.

出版信息

Curr Pharm Des. 2024;30(20):1610-1623. doi: 10.2174/0113816128304460240408085736.

DOI:10.2174/0113816128304460240408085736

PMID:38661036

Abstract

AIM

The study aimed to determine pharmacological effects of modified Ag nanoparticles (AgNPs).

BACKGROUND

AgNPs are considered antimicrobial agents. However, the cytotoxicity of chemically synthesized AgNPs (cAgNPs) has raised challenges that limit their use.

OBJECTIVE

The purpose of the study was to examine the antimicrobial and cytotoxicity effects of AgNPs synthesized using extract modified by chitosan/alginate AgNPS (Ch/ALG-gAgNPs).

METHODS

Nanoparticles were characterized using TEM, DLS, XRD, and FTIR. Resistant strains of were used for the antimicrobial analysis of Ch/ALG-gAgNPs using disc diffusion and microdilution methods. The effects of NPs on cell viability and apoptosis in L929 normal cells were determined using MTT assay and annexin/PI staining, respectively.

RESULTS

Physicochemical characterizations confirmed Ch/ALG-gAgNPs to be spherical and uniformly dispersed, and their size ranged from 50 to 500 nm. Ch/ALG-gAgNPs inhibited the growth of microbial strains in a dose-dependent manner. The antibacterial effect of Ch/ALG-gAgNPs was significantly higher than cAgNPs. The Ch/ALG-gAgNPs showed little cytotoxicity against normal cells at concentrations less than 50 μg/ml. Cytotoxicity effects of Ch/ALG-gAgNP were less than cAgNPs. Flow cytometry and real-time PCR results showed a decrease in apoptosis percentage and BAX marker in the presence of Ch/ALG-gAgNPs relative to when the cell was treated with cAgNPs.

CONCLUSION

Current findings introduce novel gAgNPs modified with chitosan/alginate for use in medicine.

摘要

目的

本研究旨在确定修饰后的纳米银颗粒（AgNPs）的药理作用。

背景

AgNPs 被认为是一种抗菌剂。然而，化学合成的 AgNPs（cAgNPs）的细胞毒性引发了一些挑战，限制了其应用。

目的

本研究旨在研究壳聚糖/海藻酸钠修饰提取物合成的 AgNPs（Ch/ALG-gAgNPs）的抗菌和细胞毒性作用。

方法

使用 TEM、DLS、XRD 和 FTIR 对纳米颗粒进行了表征。使用纸片扩散法和微量稀释法对 Ch/ALG-gAgNPs 的抗菌分析，使用 MTT 法和 annexin/PI 染色法分别确定 NPs 对 L929 正常细胞活力和细胞凋亡的影响。

结果

物理化学特性证实 Ch/ALG-gAgNPs 为球形且均匀分散，其粒径范围为 50 至 500nm。Ch/ALG-gAgNPs 以剂量依赖性方式抑制微生物菌株的生长。Ch/ALG-gAgNPs 的抑菌效果明显高于 cAgNPs。Ch/ALG-gAgNPs 在浓度小于 50μg/ml 时对正常细胞几乎没有细胞毒性。Ch/ALG-gAgNP 的细胞毒性作用小于 cAgNPs。流式细胞术和实时 PCR 结果表明，与用 cAgNPs 处理的细胞相比，Ch/ALG-gAgNPs 存在时，细胞凋亡百分比和 BAX 标志物减少。