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L.乙醇提取物在急性咽炎大鼠模型中的抗咽炎作用及环烯醚萜苷类化合物在脂多糖诱导的RAW 264.7细胞中的抗炎作用

Antipharyngitis Effects of L. Ethanolic Extract in Acute Pharyngitis Rat Model and Anti-Inflammatory Effect of Ir-Idoids in LPS-Induced RAW 264.7 Cells.

作者信息

Zhu Wen-Bo, Su Fa-Zhi, Sun Yan-Ping, Yang Bing-You, Wang Qiu-Hong, Kuang Hai-Xue

机构信息

Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, China.

School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, 232 Outer Ring Road, University Town, Guangzhou 510006, China.

出版信息

Evid Based Complement Alternat Med. 2021 Dec 10;2021:5111752. doi: 10.1155/2021/5111752. eCollection 2021.

DOI:10.1155/2021/5111752
PMID:34925529
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8683189/
Abstract

Acute pharyngitis is an inflammation of the pharyngeal mucous membrane and submucous lymphoid tissues. Unsatisfactory treatment and repeated occurrences might cause chronic pharyngitis and other complications. L. () is a traditional Chinese medicine that exhibited a significant therapeutic effect on various inflammatory diseases. Its commercial drug Yan Li Xiao (YLX) capsule is used as a cure for the treatment of inflammatory diseases, such as bacillary dysentery, tonsillitis, bronchitis, and acute gastroenteritis. However, studies about relieving acute pharyngitis are rare. In this study, high-performance liquid chromatography (HPLC) was used to analyze the chemical profile of and the bioactive phytoconstituents were isolated and identified by nuclear magnetic resonance (NMR) and mass spectrometry. An ammonia-induced acute pharyngitis rat model was established to estimate the protective effect of in vivo for the first time. The severity of pharyngitis was observed by appearance index and HE staining. The cytokines expression was performed by ELISA. Crucial proteins expression associated with TLR4/NF-B/MAPK and NLRP3 inflammasome signaling pathways were analyzed by western blotting and immunohistochemistry. Furthermore, the anti-inflammatory effect of isolated compounds was evaluated by suppressing lipopolysaccharide- (LPS-) induced NO generation and regulating the cytokines levels in RAW 264.7 cells. The results showed that exhibited a protective effect in the ammonia-induced acute pharyngitis rat model, and the compounds exert potential anti-inflammatory properties against LPS-activated RAW 254.7 cells.

摘要

急性咽炎是咽黏膜及黏膜下淋巴组织的炎症。治疗不彻底和反复发病可能会导致慢性咽炎及其他并发症。[此处“L. ()”原文有误，推测可能是某种中药材名称，暂按原文翻译]是一种对多种炎症性疾病具有显著治疗作用的传统中药。其商业药物咽立消（YLX）胶囊用于治疗炎症性疾病，如细菌性痢疾、扁桃体炎、支气管炎和急性肠胃炎。然而，关于缓解急性咽炎的研究很少。在本研究中，采用高效液相色谱法（HPLC）分析[此处“ and”原文有误，推测可能是某种中药材名称，暂按原文翻译]的化学图谱，并通过核磁共振（NMR）和质谱法分离鉴定生物活性植物成分。首次建立氨诱导的急性咽炎大鼠模型以评估[此处“ ”原文有误，推测可能是某种中药材名称，暂按原文翻译]在体内的保护作用。通过外观指数和苏木精-伊红（HE）染色观察咽炎的严重程度。采用酶联免疫吸附测定（ELISA）法检测细胞因子表达。通过蛋白质免疫印迹法和免疫组织化学法分析与Toll样受体4/核因子-κB/丝裂原活化蛋白激酶（TLR4/NF-κB/MAPK）和NOD样受体蛋白3（NLRP3）炎性小体信号通路相关的关键蛋白表达。此外，通过抑制脂多糖（LPS）诱导的一氧化氮（NO）生成和调节RAW 264.7细胞中的细胞因子水平来评估分离化合物的抗炎作用。结果表明，[此处“ ”原文有误，推测可能是某种中药材名称，暂按原文翻译]在氨诱导的急性咽炎大鼠模型中具有保护作用，并且这些化合物对LPS激活的RAW 254.7细胞具有潜在的抗炎特性。

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