Miao Mingsan, Peng Mengfan, Liu Baosong, Bai Ming
College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Saudi J Biol Sci. 2019 May;26(4):816-820. doi: 10.1016/j.sjbs.2019.02.008. Epub 2019 Feb 20.
Explore the effect of compound lobelia oral liquid on acute pharyngitis rabbits model caused by ammonium hydroxide, evaluate its curative effect.
Acute pharyngitis rabbit model was prepared by ammonia spray at pharyngeal. After 1 h of the last administration, score the pharyngeal tissue symptoms of rabbits under the condition of naked eyes (score according to the score criteria). Then, take blood from the marginal auricular vein for routine blood tests and serum TNF-α level measure. Take pharyngeal tissue for HE staining, and observe its pathological changes under the microscope.
Compared with the blank group, the pharyngeal tissue symptom score and serum TNF-α level in the model group were significantly increased ( < 0.01). The results of blood routine showed that the number of white blood cells and lymphocytes in the model group increased significantly ( < 0.01), and neutrophilic granulocyte percentage decreased significantly ( < 0.01). The infiltration of inflammatory cells in the pharynx tissue was obvious and pathological changes were observed. Compared with the model group, large, middle and small doses of compound lobelia oral liquid group could reduce the scores of pharyngeal tissue symptoms in rabbits with acute pharyngitis induced by ammonia. The large and middle dose compound lobelia oral liquid group could significantly reduce serum TNF-α level and neutrophilic granulocyte percentage, significantly increase the number of white blood cells and lymphocytes ( < 0.01); The small dose compound lobelia oral liquid group could significantly reduce the number of white blood cells and lymphocytes ( < 0.01), there was an increasing tendency to neutrophils and a decreasing tendency to serum TNF-α, but no statistical significance. The large, middle and small doses of compound lobelia oral liquid group all could improve the pathological changes of pharyngeal tissue inflammatory infiltration.
Compound lobelia oral liquid has a good effect on rabbit acute pharyngitis model caused by ammonia.
探讨复方半边莲口服液对氢氧化铵所致急性咽炎家兔模型的影响，评价其疗效。
采用咽部喷氨法制备急性咽炎家兔模型。末次给药1小时后，肉眼观察家兔咽部组织症状并评分（按评分标准评分）。然后，从耳缘静脉取血进行血常规检查及血清TNF-α水平测定。取咽部组织进行HE染色，显微镜下观察其病理变化。
与空白组比较，模型组咽部组织症状评分及血清TNF-α水平显著升高（<0.01）。血常规结果显示，模型组白细胞及淋巴细胞数量显著增加（<0.01），中性粒细胞百分比显著降低（<0.01）。咽部组织有明显的炎性细胞浸润并观察到病理变化。与模型组比较，大、中、小剂量复方半边莲口服液组均可降低氨致急性咽炎家兔的咽部组织症状评分。大、中剂量复方半边莲口服液组可显著降低血清TNF-α水平及中性粒细胞百分比，显著增加白细胞及淋巴细胞数量（<0.01）；小剂量复方半边莲口服液组可显著降低白细胞及淋巴细胞数量（<0.01），中性粒细胞有增加趋势，血清TNF-α有降低趋势，但无统计学意义。大、中、小剂量复方半边莲口服液组均可改善咽部组织炎性浸润的病理变化。
复方半边莲口服液对氨致家兔急性咽炎模型有良好疗效。
