利用白藜芦醇脂质体作为一种无抗生素的治疗策略重塑牙周炎中的免疫微环境。
Remodeling immune microenvironment in periodontitis using resveratrol liposomes as an antibiotic-free therapeutic strategy.
机构信息
Department of Implant Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of MedicineCollege of Stomatology, Shanghai Jiao Tong University; National Center for Stomatology, National Clinical Research Center for Oral Diseases; Shanghai Key Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai, 200011, China.
State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 501 Haike Rd, Shanghai, 201203, China.
出版信息
J Nanobiotechnology. 2021 Dec 20;19(1):429. doi: 10.1186/s12951-021-01175-x.
BACKGROUND
Periodontitis is a complicated inflammatory disease that damages the tooth-supporting tissues, with limited pharmacotherapy available. Macrophage-targeting therapy is promising for inflammatory diseases. Resveratrol (RSV), a nonflavonoid polyphenol, is known for its anti-inflammatory and immunomodulatory effects. However, its medical application is limited by its poor stability and water-solubility, as well as its low bioavailability.
RESULT
A therapeutic resveratrol-loaded liposomal system (Lipo-RSV) was developed to treat periodontitis. The physical properties of Lipo-RSV and its ability to regulate macrophages were investigated. The results showed that Lipo-RSV had good biocompatibility and could re-educate the inflammatory macrophages from M1- to M2-like phenotype through activating p-STAT3 and downregulating p-STAT1. Besides, the Lipo-RSV could scavenge ROS and inhibit the NF-κB signal and inflammasomes, thereby reducing the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α.
CONCLUSION
These results revealed that Lipo-RSV could be a potential therapeutic system for the antibiotic-free treatment for periodontal diseases.
背景
牙周炎是一种复杂的炎症性疾病，会破坏牙齿支持组织，目前可用的药物治疗有限。针对炎症性疾病的巨噬细胞靶向治疗具有广阔的前景。白藜芦醇（RSV）是一种非黄酮多酚，具有抗炎和免疫调节作用。然而，其医学应用受到其较差的稳定性、水溶性以及低生物利用度的限制。
结果
开发了一种负载白藜芦醇的脂质体系统（Lipo-RSV）来治疗牙周炎。研究了 Lipo-RSV 的物理性质及其对巨噬细胞的调节作用。结果表明，Lipo-RSV 具有良好的生物相容性，可通过激活 p-STAT3 和下调 p-STAT1 将炎症性巨噬细胞从 M1 样表型重编程为 M2 样表型。此外，Lipo-RSV 可以清除 ROS 并抑制 NF-κB 信号和炎症小体，从而减少促炎细胞因子 IL-1β、IL-6 和 TNF-α。
结论
这些结果表明，Lipo-RSV 可能成为一种用于牙周病无抗生素治疗的有潜力的治疗系统。
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