Department of Cariology at the Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands,
Department of Dentistry, Comprehensive Specialized Clinics and Hospital of Security Forces, Mecca City, Saudi Arabia,
Caries Res. 2022;56(1):64-72. doi: 10.1159/000521453. Epub 2021 Dec 22.
This study compared the effect of topically applied fluoride products on dentine lesions in an in vitro experiment. Demineralized bovine dentine specimens were treated once with either SDF solution (35,400 ppm F), NaF varnish (22,600 ppm F), TiF4 solution (9,200 ppm F), SnF2 gel (1,000 ppm F), no treatment (control), or preserved as baseline lesions. After the application and subsequent removal of the fluoride products, the specimens were subjected to pH-cycling. Calcium loss and uptake in the de- and remineralization buffers were assessed daily. Fluoride release into the buffers was analyzed on days 1, 2, 3, 5, 8, and 13. After the pH-cycling period, mineral distribution throughout the lesion depth was analyzed using transversal microradiography (TMR). X-ray energy-dispersive spectroscopy (EDS) examined the deposition of silver, titanium, and tin after application of SDF, TiF4, and SnF2, respectively. Overall, calcium loss and uptake analysis in the de- and remineralization buffers revealed that the SDF product was the most effective in inhibiting lesion progression, followed by the TiF4, NaF, and SnF2 products. Fluoride analysis disclosed a steep reduction of the amount of fluoride released into de- and remineralization buffers with time. The fluoride effects on de- and remineralization continued beyond the days that fluoride was released into the buffers. TMR analysis showed significant remineralization in the outer zone of the dentine lesions for all fluoride products, with SDF giving hypermineralization in this zone. In the inner zone, lesions developed in all fluoride groups, with the smallest in the SDF group. EDS showed silver and titanium deposition in depth up to 85 μm and 8 μm, respectively, while no tin deposition was observed. The silver in the dentine lesions did not contribute significantly to the density of the TMR profiles in the SDF group. In conclusion, all topical fluoride products protected the dentine lesions against lesion progression, but at different degrees. SDF showed a superior effect in protection against further demineralization and enhancement of remineralization. This was probably attributed to its fluoride concentration that was the highest among the fluoride products.
本研究通过体外实验比较了局部应用氟化物产品对牙本质龋损的影响。将脱矿牛牙本质标本用 SDF 溶液（35400ppmF）、NaF 漆（22600ppmF）、TiF4 溶液（9200ppmF）、SnF2 凝胶（1000ppmF）、不处理（对照）或保存为基线病变一次处理。在应用和随后去除氟化物产品后，将标本进行 pH 循环。每天评估去矿化和再矿化缓冲液中的钙损失和摄取。在第 1、2、3、5、8 和 13 天分析氟化物释放到缓冲液中。pH 循环期后，使用横切显微射线照相术（TMR）分析整个病变深度的矿物质分布。X 射线能量色散光谱（EDS）分别检查了 SDF、TiF4 和 SnF2 应用后银、钛和锡的沉积。总体而言，去矿化和再矿化缓冲液中的钙损失和摄取分析表明，SDF 产品在抑制病变进展方面最有效，其次是 TiF4、NaF 和 SnF2 产品。氟化物分析显示，随着时间的推移，释放到去矿化和再矿化缓冲液中的氟化物量急剧减少。氟化物对去矿化和再矿化的影响在氟化物释放到缓冲液的天数之外仍在继续。TMR 分析表明，所有氟化物产品均在牙本质病变的外区有明显的再矿化，SDF 在此区产生超矿化。在内区，所有氟化物组均发生病变，SDF 组病变最小。EDS 显示，银和钛分别沉积到 85μm 和 8μm 的深度，而未观察到锡沉积。牙本质病变中的银对 SDF 组 TMR 图谱的密度没有显著贡献。总之，所有局部用氟化物产品均能保护牙本质病变免受病变进展，但程度不同。SDF 在防止进一步脱矿和增强再矿化方面表现出优越的效果。这可能归因于其氟化物浓度在氟化物产品中最高。
