Department of Industrial Environmental Engineering, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Korea.
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Korea.
Biosensors (Basel). 2021 Dec 20;11(12):526. doi: 10.3390/bios11120526.
Poly(methyl methacrylate) (PMMA) has become an appealing material for manufacturing microfluidic chips, particularly for biomedical applications, because of its transparency and biocompatibility, making the development of an appropriate bonding strategy critical. In our research, we used acetic acid as a solvent to create a pressure-free assembly of PMMA microdevices. The acetic acid applied between the PMMA slabs was activated by microwave using a household microwave oven to tightly merge the substrates without external pressure such as clamps. The bonding performance was tested and a superior bond strength of 14.95 ± 0.77 MPa was achieved when 70% acetic acid was used. Over a long period, the assembled PMMA device with microchannels did not show any leakage. PMMA microdevices were also built as a serpentine 2D passive micromixer and cell culture platform to demonstrate their applicability. The results demonstrated that the bonding scheme allows for the easy assembly of PMMAs with a low risk of clogging and is highly biocompatible. This method provides for a simple but robust assembly of PMMA microdevices in a short time without requiring expensive instruments.
聚甲基丙烯酸甲酯（PMMA）因其透明性和生物相容性而成为制造微流控芯片的一种有吸引力的材料，特别是在生物医学应用中，因此开发合适的键合策略至关重要。在我们的研究中，我们使用乙酸作为溶剂，通过微波在 PMMA 薄片之间形成无压力组装。应用于 PMMA 板之间的乙酸在微波炉中被微波激活，无需夹具等外部压力即可将基底紧密结合。测试了键合性能，当使用 70%的乙酸时，获得了 14.95±0.77 MPa 的优异键合强度。在很长一段时间内，具有微通道的组装 PMMA 装置没有显示任何泄漏。还构建了 PMMA 微器件作为蛇形 2D 被动微混合器和细胞培养平台，以证明其适用性。结果表明，该键合方案允许 PMMA 轻松组装，堵塞风险低，且高度生物相容。该方法在短时间内提供了一种简单但坚固的 PMMA 微器件组装方法，无需昂贵的仪器。
