Puyo Maëlys, Scalabrino Léa, Romanet Rémy, Simonin Scott, Klein Géraldine, Alexandre Hervé, Tourdot-Maréchal Raphaëlle
Université Bourgogne Franche-Comté, Institut Agro, Université Bourgogne, INRAE, UMR PAM 1517, 21000 Dijon, France.
DIVVA (Développement Innovation Vigne Vin Aliments) Platform, UMR Procédés Alimentaires et Microbiologiques, IUVV, 2 Rue 11 Claude Ladrey, 21000 Dijon, France.
Foods. 2024 Feb 27;13(5):724. doi: 10.3390/foods13050724.
As a biological alternative to the antimicrobial action of SO, bioprotection has been proposed to winemakers as a means to limit or prevent grape musts microbial alteration. Competition for nitrogenous nutrients and for oxygen are often cited as potential explanations for the effectiveness of bioprotection. This study analyses the effect of a bioprotective strain on the growth of one strain and one strain. Bioprotection efficiency was observed only against inoculated at the two lowest concentrations. These results indicate a potential species-dependent efficiency of the bioprotective strain and a strong impact of the initial ratio between bioprotective and apiculate yeasts. The analysis of the consumption of nitrogen compounds revealed that leucine, isoleucine, lysine and tryptophan were consumed preferentially by all three strains. The weaker assimilation percentages of these amino acids observed in at 24 h growth suggest competition with that could negatively affects the growth of the apiculate yeast in co-cultures. The slowest rate of O consumption of strain, in comparison with , was probably not involved in the bioprotective effect. Non-targeted metabolomic analyses of and co-culture indicate that the interaction between both strains particularly impact lysin and tryptophan metabolisms.
作为二氧化硫抗菌作用的一种生物替代方法，生物保护已被推荐给酿酒师，作为限制或防止葡萄汁微生物变化的一种手段。对含氮营养物质和氧气的竞争常被认为是生物保护有效性的潜在解释。本研究分析了一种生物保护菌株对一种菌株和另一种菌株生长的影响。仅在两种最低浓度接种时观察到对[具体菌株]的生物保护效率。这些结果表明生物保护菌株的效率可能因物种而异，并且生物保护酵母与尖形酵母之间的初始比例有很大影响。对氮化合物消耗的分析表明，亮氨酸、异亮氨酸、赖氨酸和色氨酸被所有三种菌株优先消耗。在24小时生长时[具体菌株]中观察到的这些氨基酸较弱的同化百分比表明与[具体菌株]存在竞争，这可能对共培养中尖形酵母的生长产生负面影响。与[对比菌株]相比，[目标菌株]最慢的耗氧速率可能与生物保护作用无关。[目标菌株]和[对比菌株]共培养的非靶向代谢组学分析表明，两种菌株之间的相互作用特别影响赖氨酸和色氨酸代谢。
