Tavares Pereira Miguel, Nowaczyk Renata, Aslan Selim, Ay Serhan S, Kowalewski Mariusz P
Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich (UZH), 8057 Zurich, Switzerland.
Department of Biostructure and Animal Physiology, Division of Histology and Embryology, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, 50-366 Wroclaw, Poland.
Animals (Basel). 2021 Dec 19;11(12):3598. doi: 10.3390/ani11123598.
Maternal immunotolerance is required for the maintenance of pregnancy, in sharp contrast with the uterine pro-inflammatory activity observed during parturition in several species. Correspondingly, in the dog, increased immune signaling at term has been suggested, but a deeper understanding of the uterine immune milieu is still missing. Thus, the availability of 30 immune-related factors was assessed in utero-placental samples collected during post-implantation (days 18-25 of pregnancy) and mid-gestation (days 35-40) stages, and at the time of prepartum luteolysis. Gene expression and/or protein localization studies were employed. Samples collected from antigestagen (aglepristone)-treated dogs were further analyzed. Progression of pregnancy was associated with the downregulation of and upregulation of ( < 0.05) at mid-gestation. When compared with mid-gestation, a higher availability of several factors was observed at term (e.g., , , ). However, in contrast with natural parturition, , , , , and were upregulated after aglepristone treatment ( < 0.05), but not or ( > 0.05). Altogether, these results show an increased immune activity during canine parturition, involving, i.a., M2 macrophages, Treg and Th cells, with strong support for progesterone-mediated immunomodulation. Furthermore, differences between term and induced parturition/abortion could relate to differences in placental maturation towards parturition and/or functional traits of antigestagens.
维持妊娠需要母体免疫耐受，这与在几个物种分娩期间观察到的子宫促炎活性形成鲜明对比。相应地，在犬类中，已有人提出足月时免疫信号会增强，但对子宫免疫环境仍缺乏更深入的了解。因此，对在植入后（妊娠第18 - 25天）、妊娠中期（第35 - 40天）以及产前黄体溶解时收集的子宫胎盘样本中的30种免疫相关因子进行了评估。采用了基因表达和/或蛋白质定位研究。对从接受抗孕激素（阿格列司酮）治疗的犬类收集的样本进行了进一步分析。妊娠进展与妊娠中期 的下调和 的上调相关（<0.05）。与妊娠中期相比，足月时观察到几种因子的可用性更高（例如， 、 、 ）。然而，与自然分娩不同，阿格列司酮治疗后 、 、 、 、 和 上调（<0.05），但 或 未上调（>0.05）。总之，这些结果表明犬类分娩期间免疫活性增强，涉及M2巨噬细胞、调节性T细胞和辅助性T细胞等，有力支持了孕酮介导的免疫调节作用。此外，足月分娩与诱导分娩/流产之间的差异可能与胎盘向分娩成熟的差异和/或抗孕激素的功能特性有关。
