Morshdy Alaa Eldin M A, Al-Mogbel Mohammed S, Mohamed Mohamed E M, Elabbasy Mohamed Tharwat, Elshafee Azza K, Hussein Mohamed A
Food Control Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Ha'il University, Ha'il P.O. Box 2240, Saudi Arabia.
Foods. 2021 Dec 4;10(12):3006. doi: 10.3390/foods10123006.
is one of the most severe foodborne pathogens found in several habitats. Therefore, this study aims to investigate the antilisterial activity of different essential oils (EOs) against multidrug-resistant (MDR) strains isolated from fresh chicken meat. Our results showed that the prevalence of in the examined samples was 48%. Seventy-eight isolates were identified as . Out of these, 64.1% were categorized as MDR and were categorized in 18 patterns with 50 MDR isolates. One isolate was selected randomly from each pattern to investigate their biofilm-forming ability, resistance, and virulence genes incidence. Out of 18 MDR isolates, 88.9% showed biofilm-forming ability. Moreover, the most prevalent resistance genes were B (72%), A (67%), A (61%), and R genes (61%). However, the most prevalent virulence genes were A (94.4%), A (88.9%), B (83.3%), and A (83.3%). The antilisterial activity of EOs showed that cinnamon bark oil (CBO) was the most effective antilisterial agent. CBO activity could be attributed to the bioactivity of cinnamaldehyde which effects cell viability by increasing the bacterial cell electrical conductivity, ion leakage, and salt tolerance capacity loss. Therefore, CBO could be an effective alternative natural agent for food safety applications.
是在多个栖息地发现的最严重的食源性病原体之一。因此，本研究旨在调查不同精油（EOs）对从新鲜鸡肉中分离出的多重耐药（MDR）菌株的抗李斯特菌活性。我们的结果表明，在所检测的样本中，其流行率为48%。78株分离株被鉴定为 。其中，64.1%被归类为多重耐药，并被分为18种模式，有50株多重耐药分离株。从每种模式中随机选择一株来研究它们的生物膜形成能力、耐药性和毒力基因发生率。在18株多重耐药分离株中，88.9%表现出生物膜形成能力。此外，最常见的耐药基因是B（72%）、A（67%）、A（61%）和R基因（61%）。然而，最常见的毒力基因是A（94.4%）、A（88.9%）、B（83.3%）和A（83.3%）。精油的抗李斯特菌活性表明，肉桂皮油（CBO）是最有效的抗李斯特菌剂。CBO的活性可归因于肉桂醛的生物活性，它通过增加细菌细胞的电导率、离子泄漏和耐盐能力丧失来影响细胞活力。因此，CBO可能是食品安全应用中一种有效的天然替代剂。
