Wang Yanping, Zhang Yang, Yang Jingnan, Li Haoran, Wang Jinju, Geng Weitao
College of Food Science and Engineering, Tianjin University of Science & Technology, Tianjin 300457, China.
Foods. 2021 Dec 16;10(12):3126. doi: 10.3390/foods10123126.
Non-alcoholic fatty liver disease (NAFLD) has become a highly concerned health issue in modern society. Due to the attentions of probiotics in the prevention of NAFLD, it is necessary to further clarify their roles. In this study, the methionine and choline-deficient (MCD) diet induced NAFLD rats model were constructed and treated with strain . MA2 by intragastric administration once a day at a dose of 1 × 10 cfu/g.bw. After 56 days of the therapeutic intervention, the lipid metabolism and the liver pathological damage of the NAFLD rats were significantly improved. The content of total cholesterol (TC) and total triglyceride (TG) in serum were significantly lower than that in the NAFLD group ( < 0.05). Meanwhile, the intestinal mucosal barrier and the structure of intestinal microbiota were also improved. The villi length and the expression of claudin-1 was significantly higher than that in the NAFLD group ( < 0.05). Then, by detecting the content of LPS in the serum and the LPS-TLR4 pathway in the liver, we can conclude that Lactobacillus plantarum MA2 could reduce the LPS by regulating the gut microecology, thereby inhibit the activation of LPS-TLR4 and it downstream inflammatory signaling pathways. Therefore, our studies on rats showed that MA2 has the potential application in the alleviation of NAFLD. Moreover, based on the application of the strain in food industry, this study is of great significance to the development of new therapeutic strategy for NAFLD.
非酒精性脂肪性肝病（NAFLD）已成为现代社会高度关注的健康问题。由于益生菌在预防NAFLD方面受到关注，有必要进一步阐明它们的作用。在本研究中，构建了蛋氨酸和胆碱缺乏（MCD）饮食诱导的NAFLD大鼠模型，并用菌株MA2以1×10 cfu/g体重的剂量每天一次灌胃治疗。经过56天的治疗干预后，NAFLD大鼠的脂质代谢和肝脏病理损伤得到显著改善。血清中总胆固醇（TC）和总甘油三酯（TG）的含量显著低于NAFLD组（P<0.05）。同时，肠道黏膜屏障和肠道微生物群结构也得到改善。绒毛长度和紧密连接蛋白-1的表达显著高于NAFLD组（P<0.05）。然后，通过检测血清中LPS的含量和肝脏中LPS-TLR4途径，我们可以得出结论，植物乳杆菌MA2可以通过调节肠道微生态来降低LPS，从而抑制LPS-TLR4及其下游炎症信号通路的激活。因此，我们对大鼠的研究表明，MA2在减轻NAFLD方面具有潜在应用价值。此外，基于该菌株在食品工业中的应用，本研究对开发NAFLD的新治疗策略具有重要意义。
