Dong Airong, Ding Xuemei, Wang Jianping, Zeng Qiufeng, Bai Shiping, Xuan Yue, Li Shanshan, Qi Sharina, Bi Xiaojuan, He Chao, Zhang Keying
Animal Nutrition Institute, Key Laboratory of Animal Disease-Resistance Nutrition, Ministry of Agriculture and Rural Affairs, Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Sichuan Sundaily Farm Ecological Food Co. Ltd. Mianyang 622100, China.
Poult Sci. 2025 Apr 1;104(6):105118. doi: 10.1016/j.psj.2025.105118.
The objective of this study was to evaluate the effects of supplementing Pediococcus acidilactici CNCM I-4622 MA 18/5 M (PA) into diets with two energy levels on the production performance and intestinal function of Lohmann pink laying hens from 49 to 65 weeks of age. A total of 1400 hens were used in this study. A 2 × 2 + 1 experimental design with 2 metabolic energy levels (2700.00 kcal/kg, NE; 2625.00 kcal/kg, LE), two PA levels (0 mg/kg, 110 mg/kg), and one group with intermittent PA supplementation (LE-0/PA). The experiment comprised 5 treatments with 14 replications each, and each replication had 20 hens. The results showed that reducing the dietary energy level tended to decrease body weight (P = 0.056). However, supplementing PA in low-energy diets tended to increase body weight in 16 weeks (P = 0.063). The LE-0/PA group had higher laying rates and feed utilization rates compared to other groups (P > 0.05), maybe resulting in greater economic benefits. The LE group had significantly lower eggshell strength, Haugh unit, trypsin activity, lipase activity, and tibia breaking strength compared to the NE group (P < 0.05). Dietary supplementation with PA significantly increased eggshell strength (P < 0.05), tibia breaking strength, villus height/crypt depth ratio (VH/CD), lipase activity, and secretory immunoglobulin A (SIgA) content in the jejunum (P < 0.05). Additionally, PA supplementation significantly reduced the expression of interleukin 8 (IL-8), tumor necrosis factor-α (TNF-α), toll-like receptor 4 (TLR-4), and nuclear factor kappa-B (NF-κB) mRNA (P < 0.05). In conclusion, reducing the dietary energy level of hens can reduce the body weight and feed utilization efficiency. However, dietary PA supplementation improved body weight, reduced the expression of intestinal inflammation-related factors, and enhanced intestinal health, particularly in low-energy diets. Intermittent PA supplementation may enhance performance benefits, suggesting its potential as a strategy to optimize production performance and intestinal health in hens.
本研究的目的是评估在两种能量水平的日粮中添加嗜酸乳杆菌CNCM I - 4622 MA 18/5 M（PA）对49至65周龄罗曼粉蛋鸡生产性能和肠道功能的影响。本研究共使用了1400只母鸡。采用2×2 + 1实验设计，有2种代谢能水平（2700.00千卡/千克，NE；2625.00千卡/千克，LE）、两种PA水平（0毫克/千克，110毫克/千克），以及一组间歇性添加PA（LE - 0/PA）。实验包括5个处理组，每个处理组有14个重复，每个重复有20只母鸡。结果表明，降低日粮能量水平有使体重下降的趋势（P = 0.056）。然而，在低能量日粮中添加PA有使16周龄时体重增加的趋势（P = 0.063）。与其他组相比，LE - 0/PA组有更高的产蛋率和饲料利用率（P > 0.05），可能带来更大的经济效益。与NE组相比，LE组的蛋壳强度、哈氏单位、胰蛋白酶活性、脂肪酶活性和胫骨断裂强度显著更低（P < 0.05）。日粮中添加PA显著提高了蛋壳强度（P < 0.05）、胫骨断裂强度、绒毛高度/隐窝深度比值（VH/CD）、脂肪酶活性以及空肠中分泌型免疫球蛋白A（SIgA）含量（P < 0.05）。此外，添加PA显著降低了白细胞介素8（IL - 8）、肿瘤坏死因子 -α（TNF -α）、Toll样受体4（TLR - 4）和核因子κB（NF -κB）mRNA的表达（P < 0.05）。总之，降低母鸡日粮能量水平会降低体重和饲料利用效率。然而，日粮中添加PA可提高体重，降低肠道炎症相关因子的表达，并增强肠道健康，特别是在低能量日粮中。间歇性添加PA可能会增强性能效益，表明其作为优化蛋鸡生产性能和肠道健康策略的潜力。
